BACKGROUND Rheology experiments have been performed on the vitreous humor, a soft gel that rests inside of the eye, to study its viscoelastic behavior and underlying macromolecular structure. A significant challenge for experimentalists is preserving the macromolecular structure when removing vitreous from in vivo conditions. OBJECTIVE We have developed a novel probe-like rheometer geometry that allows us to perform shear rheology experiments on the vitreous humor in situ. The aim of this study is to assess the feasibility of the probe geometry. METHODS Creep compliance responses of silicone oils, Xanthan gum solutions, and bovine and porcine vitreous humor were measured using the probe geometry and compared to measurements performed with standard geometries. RESULTS Viscosities calculated from the creep responses of silicone oils closely match between the probe and standard geometry. Viscosities and creep compliance values of Xanthan gum measurements achieve order of magnitude agreement between the probe and standard geometry. Significant differences are detected with the probe between bovine and porcine vitreous (p<0.001). CONCLUSIONS These results suggest the probe may feasibly measure viscosities of Newtonian fluids, and correctly detect differences in the creep response of complex fluids with varying viscoelastic behaviors.

中文翻译：

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蠕变柔顺性流变，具有类似探针的圆柱几何形状。

背景技术已经在玻璃体液上进行了流变学实验，玻璃体液是一种驻留在眼睛内部的软凝胶，以研究其粘弹性行为和潜在的大分子结构。实验者面临的重大挑战是在体内条件下去除玻璃体时要保留大分子结构。目的我们开发了一种新颖的类似探针的流变仪几何形状，使我们能够在玻璃体液上进行剪切流变学实验。这项研究的目的是评估探针几何形状的可行性。方法使用探针几何形状测量硅油，黄原胶溶液以及牛和猪的玻璃体液的蠕变顺应性响应，并将其与标准几何形状进行比较。结果由硅油的蠕变响应计算得出的粘度在探针和标准几何形状之间非常匹配。黄原胶测得的粘度和蠕变柔量值在探头和标准几何形状之间达到数量级一致。用探针检测到牛和猪玻璃体之间有显着差异（p <0.001）。结论这些结果表明，该探头可以可行地测量牛顿流体的粘度，并正确地检测具有不同粘弹性行为的复杂流体的蠕变响应差异。用探针检测到牛和猪玻璃体之间有显着差异（p <0.001）。结论这些结果表明，该探头可以可行地测量牛顿流体的粘度，并正确地检测具有不同粘弹性行为的复杂流体的蠕变响应差异。用探针检测到牛和猪玻璃体之间有显着差异（p <0.001）。结论这些结果表明，该探头可以可行地测量牛顿流体的粘度，并正确地检测具有不同粘弹性行为的复杂流体的蠕变响应差异。