BACKGROUND Reperfusion injury often occurs with therapeutic intervention addressing the arterial occlusions causing acute myocardial infarction and stroke. The no-reflow phenomenon has been ascribed to leukocyte plugging and blood vessel constriction in the microcirculation. OBJECTIVE To assess possible red cell contributions to post-thrombolytic no-reflow phenomenon. METHODS Blood clots were formed by recalcifying 1 ml of citrated fresh human venous blood and then lysed by adding 1,000 units of streptokinase (SK) at several intervals within 1 hour. Red cell deformability was tested by both a microscopic photometric and a filtration technique, viscosity by a cone and plate viscometer, and erythrocyte aggregation by an optical aggregometer. RESULTS Two sampling methods were devised for the microscopic photometric test, both of which indicated increases of erythrocyte stiffness after being lysed from the clot by SK. In accompanying experiments, the viscosity, aggregation and filterability of the post-lytic erythrocytes were assessed. Results indicated increased viscosity in Ringer's, decreased aggregation index and filterability through a 5 μm pore size Nuclepore membrane. CONCLUSION Findings demonstrated that post-lytic changes in red cell deformability do occur which could contribute to the no-reflow phenomenon.

中文翻译：

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可能是红细胞对溶栓后无复流现象的加重作用。

背景技术再灌注损伤经常发生在针对动脉闭塞的治疗干预中，所述动脉闭塞引起急性心肌梗塞和中风。无回流现象归因于微循环中的白细胞堵塞和血管收缩。目的评估红细胞对溶栓后无复流现象的可能贡献。方法血凝块是通过重新计算1 ml柠檬酸新鲜人静脉血形成的，然后在1小时内以若干间隔加入1,000单位的链激酶（SK）裂解。通过显微镜光度法和过滤技术，通过锥板粘度计的粘度和通过光学凝集仪的红细胞聚集来测试红细胞的可变形性。结果为显微光度测试设计了两种采样方法，两者都表明在被SK从血凝块中溶解后，红细胞的硬度增加了。在伴随的实验中，评估了溶解后红细胞的粘度，聚集和可过滤性。结果表明，通过5μm孔径的Nuclepore膜，林格氏粘度增加，聚集指数降低和可过滤性降低。结论研究结果表明，红细胞变形能力确实发生了溶血后改变，这可能导致无回流现象。