BACKGROUND Post-crystallization dehydration methods, applying either vapor diffusion or humidity control devices, have been widely used to improve the diffraction quality of protein crystals. Despite the fact that RNA crystals tend to diffract poorly, there is a dearth of reports on the application of dehydration methods to improve the diffraction quality of RNA crystals. RESULTS We use dehydration techniques with a Free Mounting System (FMS, a humidity control device) to recover the poor diffraction quality of RNA crystals. These approaches were applied to RNA constructs that model various RNA-mediated repeat expansion disorders. CONCLUSION The method we describe herein could serve as a general tool to improve diffraction quality of RNA crystals to facilitate structure determinations.

中文翻译：

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受控脱水提高了两个 RNA 晶体的衍射质量。

背景技术应用蒸气扩散或湿度控制装置的结晶后脱水方法已被广泛用于改善蛋白质晶体的衍射质量。尽管 RNA 晶体往往衍射较差，但缺乏关于应用脱水方法提高 RNA 晶体衍射质量的报道。结果 我们使用带有自由安装系统（FMS，湿度控制装置）的脱水技术来恢复 RNA 晶体的差衍射质量。这些方法被应用于模拟各种 RNA 介导的重复扩增障碍的 RNA 结构。结论 我们在此描述的方法可以作为提高 RNA 晶体衍射质量以促进结构测定的通用工具。