BACKGROUND The venous response to elevated blood pressure (BP) is of major importance because it is closely related to the etiology of venous diseases and the competency of vein grafts. In vitro culture experiments may provide useful information on the function of vein grafts because it is easier to separate mechanical and hemodynamic effects from other systemic influences compared to in vivo experiments. OBJECTIVE To study the effects of BP elevation on wall dimensions and mechanical properties of in vitro cultured veins. METHODS Rabbit femoral veins were cultured in vitro under internal pressures of 1 to 50 mmHg for 1 week, and their wall dimensions, biomechanical properties, and histology were determined. RESULTS No significant differences were observed in internal vein diameter and wall thickness among vessels cultured at 10-50 mmHg compared to non-cultured control vessels. For an internal pressure of 10 mmHg applied to vessels during culture (equivalent to in vivo working BP), wall circumferential stress was maintained within control levels. There were no significant effects of pressure on basal tone and contractility of vascular smooth muscle and vascular compliance. CONCLUSIONS The in vitro results were essentially similar to those obtained from previous in vivo animal experiments, indicating that in vitro tissue culture techniques are applicable to studies of venous remodeling.

中文翻译：

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在升高的内部压力下体外培养的静脉的生物力学特性。

背景技术对血压升高（BP）的静脉反应非常重要，因为它与静脉疾病的病因学和静脉移植物的能力密切相关。体外培养实验可能提供有关静脉移植物功能的有用信息，因为与体内实验相比，将机械作用和血液动力学作用与其他全身性影响更容易区分开。目的研究血压升高对体外培养静脉壁的大小和力学性能的影响。方法兔股静脉在1至50 mmHg的内压下体外培养1周，并测定其壁大小，生物力学特性和组织学。结果与未培养的对照血管相比，在10-50 mmHg的培养血管内，内静脉直径和壁厚没有观察到显着差异。对于在培养期间施加到容器的10 mmHg的内部压力（相当于体内工作BP），壁周向应力保持在控制水平之内。压力对血管平滑肌的基础张力和收缩以及血管顺应性没有显着影响。结论体外结果与以前的体内动物实验所获得的结果基本相似，表明体外组织培养技术可用于静脉重塑研究。壁的周向应力保持在控制水平内。压力对血管平滑肌的基础张力和收缩以及血管顺应性没有显着影响。结论体外结果与以前的体内动物实验所获得的结果基本相似，表明体外组织培养技术可用于静脉重塑研究。壁的周向应力保持在控制水平内。压力对血管平滑肌的基础张力和收缩以及血管顺应性没有显着影响。结论体外结果与以前的体内动物实验所获得的结果基本相似，表明体外组织培养技术可用于静脉重塑研究。