Many miRNA functions have been revealed to date. Single miRNAs can participate in life processes by regulating more than one target gene, and more than one miRNA can also simultaneously act on one target mRNA. Thus, a complex regulatory network involved in many processes can be formed. Herein, the pigmentation regulation mechanism of miR-101a-3p and miR-144a-3p was studied at the cellular level by the overexpression and equal overexpression of miR-101a-3p and miR-144a-3p. Results revealed that miR-101a-3p and miR-144a-3p directly regulated the expression of microphthalmia-associated transcription factor, thereby affecting melanin synthesis. The two miRNAs with the same binding site in the same gene independently excreted each other’s function. However, the inhibitory effect of miR-144a-3p was stronger than that of miR-101-3p in alpaca melanocytes, although both decreased melanin production.

中文翻译：

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miRNA-101a-3p和miRNA-144a-3p调控与羊驼黑素细胞色素沉着关键基因的比较。

迄今为止，已经揭示了许多miRNA功能。单个miRNA可以通过调节多个靶基因来参与生命过程，并且多个miRNA也可以同时作用于一种靶mRNA。因此，可以形成涉及许多过程的复杂的监管网络。在此，通过miR-101a-3p和miR-144a-3p的过表达和相等过表达，在细胞水平研究了miR-101a-3p和miR-144a-3p的色素沉着调节机制。结果显示，miR-101a-3p和miR-144a-3p直接调节小眼症相关转录因子的表达，从而影响黑色素的合成。在相同基因中具有相同结合位点的两个miRNA相互独立地排泄彼此的功能。但是，在羊驼黑素细胞中，miR-144a-3p的抑制作用要强于miR-101-3p，