Neurons and glial cells coordinate with each other in many different aspects of nervous system development. Both types of cells are receiving multiple guidance cues to guide the neurons and glial cells to their proper final position. The lateral chordotonal organs (lch5) of the Drosophila peripheral nervous system (PNS) are composed of five sensory neurons surrounded by four different glial cells, scolopale cells, cap cells, attachment cells and ligament cells. During embryogenesis, the lch5 neurons go through a rotation and ventral migration to reach their final position in the lateral region of the abdomen. We show here that the extracellular ligand sli is required for the proper ventral migration and morphology of the lch5 neurons. We further show that mutations in the Sli receptors Robo and Robo2 also display similar defects as loss of sli, suggesting a role for Slit-Robo signaling in lch5 migration and positioning. Additionally, we demonstrate that the scolopale, cap and attachment cells follow the mis-migrated lch5 neurons in sli mutants, while the ventral stretching of the ligament cells seems to be independent of the lch5 neurons. This study sheds light on the role of Slit-Robo signaling in sensory neuron development.

中文翻译：

---

果蝇PNS中sli是感觉神经元的正确形态和迁移所必需的。

神经元和神经胶质细胞在神经系统发育的许多不同方面相互协调。两种类型的细胞都接受多个引导提示，以将神经元和神经胶质细胞引导至其正确的最终位置。果蝇外周神经系统（PNS）的外侧弦索器官（lch5）由五个感觉神经元组成，周围环绕着四个不同的神经胶质细胞，扇形细胞，帽细胞，附着细胞和韧带细胞。在胚胎发生过程中，lch5神经元经历旋转和腹侧迁移，以到达其在腹部外侧区域的最终位置。我们在这里显示胞外配体sli是lch5神经元的适当腹腔迁移和形态所必需的。我们进一步证明Sli受体Robo和Robo2中的突变也显示出与sli缺失相似的缺陷，提示Slit-Robo信号在lch5迁移和定位中的作用。此外，我们证明了扇贝，帽和附着细胞遵循sli突变体中错误迁移的lch5神经元，而韧带细胞的腹侧舒张似乎独立于lch5神经元。这项研究揭示了Slit-Robo信号在感觉神经元发育中的作用。