Centrosomes are highly conserved organelles that act as the major microtubule-organizing center (MTOC) in animal somatic cells. Through their MTOC activity, centrosomes play various roles throughout the cell cycle, such as supporting cell migration in interphase and spindle organization and positioning in mitosis. Various approaches for removing centrosomes from somatic cells have been developed and applied over the past few decades to understand the precise roles of centrosomes. Centrinone, a reversible and selective PLK4 (polo-like kinase 4) inhibitor, has recently emerged as an efficient approach to eliminate centrosomes. In this review, we describe the latest findings on centrosome function that have been revealed using various centrosome-eliminating approaches. In addition, we discuss our recent findings on the mechanism of centrosome-independent spindle bipolarization, discovered through the use of centrinone.Key words: centrosome, centrinone, mitotic spindle, bipolarity, NuMA.

中文翻译：

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通过消除中心体来实现中心体功能和非中心体功能。

中心体是高度保守的细胞器，充当动物体细胞中的主要微管组织中心（MTOC）。通过其MTOC活性，中心体在整个细胞周期中扮演着各种角色，例如支持细胞在相间和纺锤体组织中迁移以及在有丝分裂中定位。在过去的几十年中，已经开发出了多种从体细胞中去除中心体的方法，并应用了这些方法来了解中心体的确切作用。Centrinone是一种可逆的选择性PLK4（polo样激酶4）抑制剂，最近已成为消除中心体的有效方法。在这篇综述中，我们描述了使用各种消除中心体的方法揭示的有关中心体功能的最新发现。此外，