Genus Zacco specimens collected in this study were classified genetically as five species, Zacco platypus, Z. temminckii, Z. koreanus and two unidentified species, using DNA barcoding analysis based on 655 bp of mitochondrial cytochrome c oxidase subunit I (COI) gene. Two of unidentified species (Z. sp.1 and Z. sp.2) were considered to be unrecorded or new species of genus Zacco according to genetic distances between Zacco species. In addition, we determined a natural hybrid based on polymorphic base at the diagnostic positions displayed on nuclear recombination activating gene 1 (RAG1) gene (965 bp), and estimated paternal and maternal species of natural hybrid comparing phylogenetic tree between COI and RAG1, and Z. sp.1♀ × Z. koreanus♂, Z. sp.2♀ × Z. koreanus♂ and Z. koreanus♀ × Z. sp.1♂ individuals were confirmed. The habitat of natural hybrids of Z. koreanus between Z. sp.1 and Z. sp.2 was identified as Geum and Yeongsan River, respectively. In our data, only F1 hybrid generation was identified; however, generations after F1 hybrid or backcross were not demonstrated.

使用基于655 bp线粒体细胞色素C氧化酶亚基（COI）基因的DNA条形码分析，将本研究中收集的Zacco属标本遗传分类为五个物种，即鸭嘴兽（Zacco platypus），斑节菌（Z. temminckii），朝鲜蓟（Z. koreanus）和两个未鉴定物种。未鉴定物种（两个Ž。SP.1和Ž。SP.2）被认为是属的未记录的或新的物种宽鳍根据之间的遗传距离宽鳍种类。此外，我们确定显示在核重组激活基因1（基于多态性碱基在诊断位置的天然杂交RAG1）基因（965个碱基），和估计的比较之间的系统发生树的自然混合的父系和母系物种COI和RAG1，和ž。sp.1♀×  Z. koreanus♂，Z。sp.2♀×  Z.朝鲜球♂和ž。朝鲜球♀×  Z. sp.1♂个人进行了证实。自然杂交种的栖息地Z.朝鲜球之间ž。sp.1和Z。sp.2被分别确定为锦江和龙山河。在我们的数据中，仅识别出F1杂种一代。但是，没有证明F1杂交或回交后的世代。