Multi-phenotypic screening of zebrafish larvae, such as monitoring the heart and tail activities, is important in biological assays. Microfluidic devices have been developed for zebrafish phenotypic assays, but simultaneous lateral–dorsal screening of the same larva in a single chip is yet to be achieved. We present a multi-phenotypic microfluidic device for monitoring of tail movement and heart rate (HR) of 5–7-day postfertilization zebrafish larvae. Tail movements were stimulated using electric current and quantified in terms of response duration (RD) and tail beat frequency (TBF). The positioning of a right-angle prism provided a lateral view of the larvae and enabled HR monitoring. Investigations were performed on zebrafish larvae exposed to 3% ethanol, 250 μM 6-hydroxydopamine (6-OHDA) or 1 mM levodopa. Larvae exposed to ethanol showed a significant drop in HR, whereas electric stimulation increased the HR temporarily. Larvae experienced a significant drop in RD, TBF and HR when exposed to 6-OHDA. HR was not affected by levodopa post-treatment, whereas RD and TBF were restored to normal levels. The results showed potential for applications that involve monitoring of cardiac and behavioral parameters in zebrafish larvae. Tests can be done using the same chip, without changing the larvae’s orientation. This eliminates undue stress caused by reorientation, which may affect their behavior, and the use of separate devices to obtain dorsal and lateral views. The device can be implemented to improve multi-phenotypic and quantitative screening of zebrafish larvae in response to chemical and physical stimuli in different zebrafish disease models.

中文翻译：

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斑马鱼幼虫的多表型和双向行为筛选。

斑马鱼幼虫的多表型筛选，例如监测心脏和尾部活动，在生物测定中很重要。已开发出用于斑马鱼表型分析的微流体装置，但尚未实现在单个芯片中同时对同一幼虫进行侧背筛查。我们提出了一种多表型微流体装置，用于监测受精后 5-7 天斑马鱼幼虫的尾部运动和心率 (HR)。使用电流刺激尾部运动，并根据响应持续时间 (RD) 和尾拍频率 (TBF) 进行量化。直角棱镜的定位提供了幼虫的侧视图并启用了 HR 监测。对暴露于 3% 乙醇、250 μM 6-羟基多巴胺 (6-OHDA) 或 1 mM 左旋多巴的斑马鱼幼虫进行了研究。暴露于乙醇的幼虫显示 HR 显着下降，而电刺激暂时增加了 HR。当暴露于 6-OHDA 时，幼虫的 RD、TBF 和 HR 显着下降。HR 不受左旋多巴治疗后的影响，而 RD 和 TBF 恢复到正常水平。结果显示了涉及监测斑马鱼幼虫心脏和行为参数的应用潜力。可以使用相同的芯片进行测试，而无需改变幼虫的方向。这消除了由重新定向引起的过度压力，这可能会影响他们的行为，以及使用单独的设备来获得背侧和侧视图。该装置可用于改善斑马鱼幼虫对不同斑马鱼疾病模型中化学和物理刺激的多表型和定量筛选。