Photoacoustic spectroscopy has been used to measure optical absorption coefficient and the application of tens of wavelength bands in photoacoustic spectroscopy was reported. Using optical methods, absorption-related information is, generally, derived from reflectance or transmittance values. Hence measurement accuracy is limited for highly absorbing samples where the reflectance or transmittance is too low to give reasonable signal-to-noise ratio. In this context, this paper proposes and illustrates a hyperspectral photoacoustic spectroscopy system to measure the absorption-related properties of highly absorbing samples directly. The normalized optical absorption coefficient spectrum of the highly absorbing iris is acquired using an optical absorption coefficient standard. The proposed concepts and the feasibility of the developed diagnostic medical imaging system are demonstrated using fluorescent microsphere suspensions and porcine eyes as test samples.

光声光谱法已被用于测量光吸收系数，并且据报道在光声光谱法中数十个波长带的应用。使用光学方法，通常从反射率或透射率值得出与吸收有关的信息。因此，对于反射率或透射率太低而无法给出合理信噪比的高吸收样品，测量精度受到限制。在此背景下，本文提出并举例说明了一种高光谱光声光谱系统，用于直接测量高吸收样品的吸收相关特性。使用光吸收系数标准来获取高吸收虹膜的归一化光吸收系数谱。