Abstract

The work was aimed to investigate earthworms species particularly Eutyphoeus endemic to India with the use of DNA barcodes and usual morpho-anatomical standards of earthworm taxonomy from protected areas of Garo Hills, Meghalaya, the north-east region (NER) of India. The study revealed two new records Eutyphoeus kempi Stephenson, E. nepalensis Michaelsen and confirms availability of three known sp. namely E. callosus Gates, E. gammiei Beddard and E. turaensis Stephenson. The neighbor-joining tree was constructed using the K2P substitution model and the genomic signature of each species using COI-1 gene was generated for the first time and were used to reconfirm the identification of species.

摘要

这项工作旨在利用DNA条形码和印度东北部（Meghalaya）加罗山（Gero Hills）保护区的usual分类学通常的形态解剖标准，研究印度特有的Eu类。该研究揭示了两个新纪录Eutyphoeus蛄斯蒂芬森，E.冬瓜三个已知SP的Michaelsen并确认可用性。即E. callosus盖茨E. gammiei Beddard和E. turaensis斯蒂芬森。使用K2P替换模型构建邻居连接树，并使用COI-1构建每个物种的基因组签名 该基因是首次产生，并用于再次确认物种鉴定。