Objective: Conventional microbiological methods (CMM), including long-term culture, for the diagnosis of osteo-articular infections (OAI) fail in at least 5% of all cases. Only one IOA dedicated molecular method has been commercialized, and only the first version of this kit has been studied. The aim of this work was to evaluate the concordance between test results obtained with the second version of the Unyvero ITI G2 cartridge (Curetis) and CMM. The cartridge, combining one-step automated lysis/DNA extraction with multiplex PCR and amplicon detection by array hybridization, allows for the detection of 102 prevalent pathogens and their antibiotic resistance markers directly in clinical specimens (liquid (n=8) or solid (n=32)).

Material and methods: Frozen samples from 40 patients who underwent orthopedic surgery at Pitié-Salpêtrière hospital were tested retrospectively with the cartridge: 5 were culture-negative, 25 revealed monomicrobial and 10 polymicrobial OAI. The 2 main surgical sites were hip (22.5%) and knee (17.5%).

Results: Extraction, amplification and hybridization reactions were completed in 28 of the 40 cases, failed in all cartridge chambers in 6 cases, and in 1 or 2 chambers in an additional 6 cases. Overall sensitivity and specificity for microorganism identification were estimated at 67.6% and 98.2%, when complete and partial failures were excluded.

Conclusions: These results show that the performances of the second version of the Unyvero ITI G2 cartridge should be further enhanced before considering avoiding conventional microbiological methods.

目的：用于诊断骨关节感染 (OAI) 的传统微生物学方法 (CMM)，包括长期培养，在所有病例中至少有 5% 失败。只有一种 IOA 专用分子方法已经商业化，并且只研究了该试剂盒的第一个版本。这项工作的目的是评估使用第二版 Unyvero ITI G2 墨​​盒 (Curetis) 和 CMM 获得的测试结果之间的一致性。该试剂盒将一步自动裂解/DNA 提取与多重 PCR 和通过阵列杂交的扩增子检测相结合，可以直接检测临床标本（液体（n=8）或固体（n=8）中的 102 种流行病原体及其抗生素抗性标志物） =32))。

材料和方法：对 Pitié-Salpêtrière 医院接受骨科手术的 40 名患者的冷冻样本进行了回顾性检测：5 名培养阴性，25 名显示单一微生物和 10 名多微生物 OAI。2 个主要手术部位是髋关节 (22.5%) 和膝关节 (17.5%)。

结果： 40例中28例完成提取、扩增和杂交反应，6例全部盒室失败，另外6例1或2室失败。在排除完全和部分失败后，微生物鉴定的总体灵敏度和特异性估计为 67.6% 和 98.2%。

结论：这些结果表明，在考虑避免传统微生物方法之前，应进一步提高 Unyvero ITI G2 墨​​盒第二版的性能。