The biocompatible polyurethane acrylate (PUA) nanopillars were fabricated by soft lithography using three different sizes of nanobeads (350, 500, and 1000 nm), and the human adipose-derived stem cells (hASCs) were cultured on the nanopillars. The hASCs and their various behaviors, such as cytoplasmic projections, migration, and morphology, were observed by high resolution images using a scanning electron microscope (SEM). With the accurate analysis by SEM for the controlled sizes of nanopillars, the deflections are observed at pillars fabricated with 350- and 500-nm nanobeads. These high-resolution images could offer crucial information to elucidate the complicated correlations between nanopillars and the cells, such as morphology and cytoplasmic projections.

中文翻译：

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通过高分辨率扫描电子显微镜图像分析纳米柱周期性阵列上的干细胞行为

使用三种不同尺寸的纳米珠（350、500 和 1000 nm）通过软光刻制造生物相容性聚氨酯丙烯酸酯 (PUA) 纳米柱，并在纳米柱上培养人类脂肪干细胞 (hASC)。使用扫描电子显微镜 (SEM) 通过高分辨率图像观察 hASC 及其各种行为，例如细胞质投影、迁移和形态。通过 SEM 对受控尺寸的纳米柱进行准确分析，在用 350 和 500 nm 纳米珠制造的柱子上观察到偏转。这些高分辨率图像可以提供重要信息来阐明纳米柱与细胞之间的复杂相关性，例如形态学和细胞质投影。