Abstract

Fishes are an important group of vertebrates in the animal world and make a significant contribution to global biodiversity. Fish is used as a source of food and contains many essential vitamins and fatty acids. The study of fish and their stability is important because, from year to year, fish stocks are often very important. For the conservation and management of these dwindling resources, correct identification of species is a prerequisite. Character-based methods of identification are of considerable use in this context, which classify specimens into species using classification rules that compactly describe species in terms of key diagnostic nucleotides in the gene sequences chosen. In this study, a total of 56 species of fishes distributed in Pulicat lake waters is taken as the target group. Mitochondrial CO1 sequences of each species were downloaded and modified. The species-specific diagnostic nucleotides for the selected group of species were identified using the BLOG version 2.0 software. Species-specific probes with a length range of 18–37 bp were designed on the basis of identified diagnostic nucleotide sites. The method is an effective tool for quickly and easily obtaining a significant amount of reliable information and could be used for forensic applications and conservation of fishes in Pulicat Lake.

摘要

鱼类是动物界重要的脊椎动物群，对全球生物多样性做出了重大贡献。鱼被用作食物来源，含有许多必需的维生素和脂肪酸。对鱼类及其稳定性的研究很重要，因为年复一年，鱼类种群通常非常重要。为了保护和管理这些日益减少的资源，正确识别物种是先决条件。在这种情况下，基于特征的鉴定方法非常有用，它使用分类规则将标本分类为物种，这些分类规则根据所选基因序列中的关键诊断核苷酸来紧凑地描述物种。本研究以分布于普利卡特湖水域的56种鱼类为研究对象。下载并修改了每个物种的线粒体 CO1 序列。使用 BLOG 2.0 版软件确定了所选物种组的物种特异性诊断核苷酸。基于已确定的诊断核苷酸位点设计了长度范围为 18-37 bp 的物种特异性探针。该方法是一种快速、轻松地获取大量可靠信息的有效工具，可用于普里卡特湖的法医应用和鱼类保护。