ABSTRACT: We analysed prokaryotic and eukaryotic communities across the seawater pre-treatment system of Penneshaw (Kangaroo Island, South Australia) desalination plant, using 16S and 18S rRNA gene sequencing. The richness of operational taxonomic units increased downstream of the pre-treatment system (reverse osmosis feedwater) compared to raw seawater for Archaea, while it decreased for bacteria and protists. Overall, the reverse osmosis feedwater was found to be enriched in ammonia-oxidising bacteria and Archaea compared to raw seawater, and also contained greater proportions of taxa typically observed in aquatic biofilms and/or within other water treatment systems. Although the microbial load was reduced by the pre-treatment system, the increase in proportion of biofilm-associated microbes suggests the presence of active microbial communities within multimedia filters and other parts of the pre-treatment system that might increase biofouling risks.

中文翻译：

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海水淡化厂内海水预处理过程中微生物群落的变化

摘要：我们使用16S和18S rRNA基因测序分析了Penneshaw（南澳大利亚州袋鼠岛）海水淡化厂的海水预处理系统中的原核和真核生物群落。与原始海水相比，古细菌的操作分类单位的丰富度在预处理系统的下游（反渗透给水）增加了，而细菌和原生生物的丰富度则降低了。总体而言，发现反渗透给水富含氨氧化细菌和古细菌。相较于原海水，它还包含通常在水生生物膜中和/或在其他水处理系统中观察到的分类单元。尽管预处理系统降低了微生物负荷，但生物膜相关微生物比例的增加表明多媒体过滤器和预处理系统其他部分中存在活性微生物群落，这可能会增加生物污染的风险。