A modern development discusses the synthesis and validity of simple, sensitive, and versatile spectrophotometric methods for Bi(III) and Al(III) determination in pharmaceutical formulations have been conducted. In the present paper, 4-(4 acetamidophenylazo) pyrogallol has been synthesized as a new organic compound, 4-APAP, by coupling pyrogallol in a regulated pH medium with diazotized p-aminoacetanilide. 4-APAP was identified by methods of FT-IR, 1H-NMR, 13C-NMR, and thermal analysis (thermogravimetry and differential scanning calorimetry). Solvatochromic activity was also studied in solvents with different polarities. The Kamlet and Taft linear solvation energy relationship was used to correlate shifts in UV-Visible spectra of 4-APAP with Kamlet-Taft parameters (α, β, and π*). The optimum assay conditions showed linearity from 0.3–13 to 0.5–11 μg·mL −1 for Bi(III) and Al(III), respectively. Molar absorptivity values were 3.365 × 10 4 and 0.356 × 10 4 L·mol −1 ·cm −1 for Bi(III) and Al(III), with similar Sandell's sensitivity measures of 0.006 and 0.008 μg·cm −2 . Detection limits and quantification limits were 0.013 and 0.043 μg·mL −1 for Bi(III), respectively, and 0.018 and 0.059 μg·mL −1 for Al(III) with the relative standard deviation for determination of both metal ions using 4-APAP probe being <2.0%. The validity, accuracy, and efficiency of the approaches were demonstrated by the determination of Bi(III) and Al(III) in different formulations.

中文翻译：

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新型有机探针4-（4-乙酰氨基苯基偶氮）邻苯三酚的合成，用于分光光度法测定药物样品中的Bi（III）和Al（III）

现代发展探讨了用于药物制剂中Bi（III）和Al（III）测定的简单，灵敏和通用的分光光度法的合成和有效性。在本文中，通过在调节pH介质中将邻苯三酚与重氮化的对氨基乙酰苯胺偶联，合成了4-（4-乙酰氨基苯偶氮）邻苯三酚作为一种新的有机化合物4-APAP。通过FT-IR，1H-NMR，13C-NMR和热分析（热重分析和差示扫描量热法）鉴定4-APAP。在不同极性的溶剂中也研究了溶剂变色活性。使用Kamlet和Taft线性溶剂化能量关系，将4-APAP的UV-可见光谱的位移与Kamlet-Taft参数（α，β和π*）相关联。最佳测定条件显示线性范围为0.3–13至0。Bi（III）和Al（III）分别为5-11μg·mL -1。Bi（III）和Al（III）的摩尔吸收率值为3.365×10 4和0.356×10 4 L·mol -1·cm -1，相似的Sandell灵敏度测量值为0.006和0.008μg·cm -2。Bi（III）的检出限和定量限分别为0.013和0.043μg·mL -1，Al（III）的检出限和定量限为0.018和0.059μg·mL -1，其中相对标准偏差用于使用4-测定两种金属离子APAP探针小于2.0％。通过测定不同配方中的Bi（III）和Al（III），证明了该方法的有效性，准确性和效率。Bi（III）的检出限和定量限分别为0.013和0.043μg·mL -1，Al（III）的检出限和定量限为0.018和0.059μg·mL -1，其中相对标准偏差用于使用4-测定两种金属离子APAP探针小于2.0％。通过测定不同配方中的Bi（III）和Al（III），证明了该方法的有效性，准确性和效率。Bi（III）的检出限和定量限分别为0.013和0.043μg·mL -1，Al（III）的检出限和定量限为0.018和0.059μg·mL -1，其中相对标准偏差用于使用4-测定两种金属离子APAP探针小于2.0％。通过测定不同配方中的Bi（III）和Al（III），证明了该方法的有效性，准确性和效率。