Introduction: Poliovirus causes paralysis by infecting the nervous system. Currently, 2 types of polio vaccine are given in many countries in polio eradication program including inactivated polio vaccine (IPV) and oral polio vaccine (OPV). Because of OPV-related paralysis, OPV should be replaced by IPV. Methods: The aim of this study was to prepare the gamma-irradiated IPV and determine its effectiveness compared with the commercial vaccine (OPV) in the mouse model. The virus titration of OPV was determined and then inactivated by the appropriate dose of gamma radiation into an irradiated vaccine formula. The vaccine was inoculated in BALB/c mice in 2 different formulations of intramuscular injection with 2-week intervals. The level of anti-polio-neutralizing antibody and polio-specific splenocyte proliferation assay were evaluated by collecting the blood samples and spleens of the vaccinated groups with conventional vaccine and irradiated vaccine. Results: There was a significant increase in the neutralizing antibody titration between all of the vaccinated groups and negative control group (A) (p #x3c; 0.05). And it shows that the IPV by gamma irradiation has the highest antibody titration. Also, the increasing of stimulation index value in the B* group, F group, and G group was the most against other groups. Furthermore, the neutralizing anti-serum titer and splenic lymphocyte proliferation assay show humoral and cellular immunity were significantly increased in the irradiated vaccine group as compared with conventional group. Conclusion: According to the results, gamma-irradiated IPV could induce humoral and cellular immunity in vaccinated mouse groups, so the irradiated poliovirus could be recommended as a good candidate vaccine to prevent the transport of poliovirus to the central nervous system and thus protect against paralysis.
Intervirology

中文翻译：

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在小鼠模型中制备加海藻糖作为蛋白质稳定剂的伽马辐射灭活二价脊髓灰质炎疫苗的免疫反应

简介：脊髓灰质炎病毒通过感染神经系统导致瘫痪。目前，许多国家在脊髓灰质炎根除计划中提供两种类型的脊髓灰质炎疫苗，包括灭活脊髓灰质炎疫苗 (IPV) 和口服脊髓灰质炎疫苗 (OPV)。由于OPV相关的瘫痪，OPV应该被IPV取代。方法：本研究的目的是制备经 γ 辐照的 IPV，并在小鼠模型中确定其与商业疫苗 (OPV) 相比的有效性。确定 OPV 的病毒滴度，然后通过将适当剂量的伽马辐射灭活到辐照疫苗配方中。将疫苗以 2 种不同的肌肉注射剂型以 2 周的间隔接种到 BALB/c 小鼠中。通过收集常规疫苗和辐照疫苗接种组的血液样本和脾脏，评估抗脊髓灰质炎中和抗体水平和脊髓灰质炎特异性脾细胞增殖试验。结果：所有疫苗接种组和阴性对照组（A）之间的中和抗体滴度显着增加（p#x3c; 0.05）。并且表明伽马辐照的IPV具有最高的抗体滴定度。此外，B*组、F组和G组刺激指数值的增加与其他组相比最大。此外，中和抗血清滴度和脾淋巴细胞增殖试验表明，与常规组相比，辐照疫苗组的体液免疫和细胞免疫显着增加。结论：根据结果​​，γ-辐照 IPV 可诱导接种小鼠的体液免疫和细胞免疫，因此可推荐辐照脊髓灰质炎病毒作为一种良好的候选疫苗，以防止脊髓灰质炎病毒向中枢神经系统转运，从而预防麻痹。 .
病毒学