Genetic variations in the host TLRs genes play an important role in susceptibility and/or resistance to visceral leishmaniasis by altering the host-pathogen interaction. In this study, we investigated the association between polymorphisms of TLR4 (Asp299Gly, Thr399Ile) and TLR-9 (T-1237C), with susceptibility to visceral leishmaniasis. A bi-directional PCR amplification of specific alleles technique was used to characterize the distribution of TLR4 (Asp299Gly and Thr399Ile) and TLR9 (T-1237C) polymorphisms. A total of 60 samples were randomly selected from confirmed visceral leishmaniasis patients and 24 endemic healthy volunteers. The samples were genotyped and allele frequencies were determined. We observed that TLR4 Asp299Gly and Thr399Ile genotypes were more frequent in visceral leishmaniasis patients (10% and 15% respectively) compared to controls (4.2% and 8.3% respectively). However, the differences were not significant in TLR4 Asp299Gly and Thr399Ile alleles and genotypes. In the case of TLR9, we observed the frequency of T1237C genotype was higher in visceral leishmaniasis patients (43.3%) than in healthy controls (33.3%). Statistically significant differences were observed in TLR9 T1237C alleles and genotypes. We concluded that TLR9 T1237C, but not TLR4, gene polymorphisms can be regarded as contributors to visceral leishmaniasis susceptibility among the Indian population of Bihar state.

宿主 TLRs 基因的遗传变异通过改变宿主-病原体相互作用在对内脏利什曼病的易感性和/或抗性中发挥重要作用。在这项研究中，我们调查了 TLR4 (Asp299Gly、Thr399Ile) 和 TLR-9 (T-1237C) 多态性与内脏利什曼病易感性之间的关联。使用特定等位基因的双向 PCR 扩增技术来表征 TLR4（Asp299Gly 和 Thr399Ile）和 TLR9（T-1237C）多态性的分布。从确诊的内脏利什曼病患者和24名地方病健康志愿者中随机抽取60份样本。对样品进行基因分型并确定等位基因频率。我们观察到，与对照组（分别为 4.2% 和 8.3%）相比，TLR4 Asp299Gly 和 Thr399Ile 基因型在内脏利什曼病患者中更为常见（分别为 10% 和 15%）。然而，TLR4 Asp299Gly 和 Thr399Ile 等位基因和基因型的差异不显着。在 TLR9 的情况下，我们观察到 T1237C 基因型的频率在内脏利什曼病患者（43.3%）中高于健康对照组（33.3%）。在 TLR9 T1237C 等位基因和基因型中观察到统计学上的显着差异。我们得出结论，TLR9 T1237C，但不是 TLR4，基因多态性可被视为比哈尔邦印度人口中内脏利什曼病易感性的贡献者。在 TLR9 的情况下，我们观察到 T1237C 基因型的频率在内脏利什曼病患者（43.3%）中高于健康对照组（33.3%）。在 TLR9 T1237C 等位基因和基因型中观察到统计学上的显着差异。我们得出结论，TLR9 T1237C，但不是 TLR4，基因多态性可被视为比哈尔邦印度人口中内脏利什曼病易感性的贡献者。在 TLR9 的情况下，我们观察到 T1237C 基因型的频率在内脏利什曼病患者（43.3%）中高于健康对照组（33.3%）。在 TLR9 T1237C 等位基因和基因型中观察到统计学上的显着差异。我们得出结论，TLR9 T1237C，但不是 TLR4，基因多态性可被视为比哈尔邦印度人口中内脏利什曼病易感性的贡献者。