Background

Voriconazole (VRC) is widely recommended as the first-line therapy for invasive aspergillosis. However, surveillance studies have demonstrated that there is an increase in the frequency of azole resistance among Aspergillus fumigates isolates. In recent years, more studies on effective synergisms between natural agents and antifungal drugs have been published.

Aims

To evaluate the synergistic antifungal effect of glabridin (Gla) and VRC against A. fumigatus isolates.

Methods

Potential interactions between Gla and VRC were studied by using a microdilution checkerboard method based on the CLSI reference technique. To assess the interaction of drugs the fractional inhibitory concentration index (FICI) was calculated based on the Loewe Additivity model.

Results

The minimum inhibitory concentrations (MIC) obtained with Gla alone were relatively high (MIC₅₀ 16 μg/ml). However, our results showed synergistic interaction between Gla and VRC against A. fumigatus strains, with FICI range values between 0.15 and 0.5.

Conclusions

Synergistic activity of Gla and VRC against both VRC-sensitive and -resistant A. fumigatus isolates may lead to design new antifungal agents, especially for inhibiting those azole-resistant strains.

中文翻译：

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光甘草定和伏立康唑对烟曲霉分离株的体外相互作用

背景

伏立康唑 (VRC) 被广泛推荐为侵袭性曲霉病的一线治疗药物。然而，监测研究表明，熏烟曲霉分离株中唑类耐药性的频率增加。近年来，发表了更多关于天然药物与抗真菌药物之间有效协同作用的研究。

宗旨

评估光甘草定 (Gla) 和 VRC 对烟曲霉分离株的协同抗真菌作用。

方法

使用基于 CLSI 参考技术的微稀释棋盘法研究 Gla 和 VRC 之间的潜在相互作用。为了评估药物的相互作用，基于 Loewe Additivity 模型计算了抑制浓度分数指数 (FICI)。

结果

单独使用 Gla 获得的最小抑制浓度 (MIC) 相对较高 (MIC ₅₀ 16  μg/ml)。然而，我们的结果显示 Gla 和 VRC 之间对烟曲霉菌株的协同相互作用，FICI 范围值在 0.15 和 0.5 之间。

结论

Gla 和 VRC 对 VRC 敏感和耐药的烟曲霉分离株的协同活性可能导致设计新的抗真菌剂，特别是用于抑制那些耐唑菌株。