Background

The fungi present in the decaying remains enable a better understanding of the processes of decomposition after death. There are not many studies about fungi on decaying bodies and it is not known which fungal sampling methods are effective.

Aims

The main objective of this study was to find the best method for sampling fungi in carcasses, prove the effectiveness of this method and identify the fungal colonies in animal carcasses from experimental burials.

Methods

Samples from 13 carcasses of Sus scrofa domestica, from the experimental project Taphos-m, were taken with different materials: spatula, sterile swabs and RODAC contact plates.

Results

RODAC contact plates with the RBA culture medium showed higher proliferation of fungal colonies. Thirty genera of fungi were isolated from different substrates (bone, tissue, lime). Most of the fungi genera or groups identified have been described before in the literature, but the substrates they came from were different in some cases.

Conclusions

Sampling with RODAC contact plates was found to be the most effective method, as it provides a nutritional culture medium that may allow growth since the moment of sampling. Fungi colonies grew better in RBA culture medium because bacterial growth is inhibited. Most of the observed fungi are related to the environment but some others have been found related to decomposing bodies for the first time.

中文翻译：

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Taphos-m 中实验墓葬的埋藏学：真菌的作用

背景

腐烂的遗骸中存在的真菌使人们能够更好地了解死后的分解过程。关于腐烂尸体上真菌的研究并不多，也不知道哪种真菌取样方法是有效的。

宗旨

本研究的主要目的是寻找对尸体进行真菌取样的最佳方法，证明该方法的有效性，并从实验墓葬中鉴定动物尸体中的真菌菌落。

方法

来自实验项目 Taphos-m 的13 具Sus scrofadomestica尸体的样本使用不同的材料：抹刀、无菌拭子和 RODAC 接触板。

结果

具有 RBA 培养基的 RODAC 接触板显示出更高的真菌菌落增殖。从不同的基质（骨、组织、石灰）中分离出 30 个真菌属。大多数已鉴定的真菌属或组以前在文献中已有描述，但在某些情况下它们来自的底物不同。

结论

发现使用 RODAC 接触板取样是最有效的方法，因为它提供了一种营养培养基，可以从取样开始就允许生长。真菌菌落在 RBA 培养基中生长得更好，因为细菌生长受到抑制。大多数观察到的真菌与环境有关，但其他一些真菌首次被发现与分解尸体有关。