MATERIALS AND METHODS Quantitative expression of the RNA of these 17 genes in normal and cancerous tissues obtained using chip arrays from the public functional genomics data repository, Gene Expression Omnibus (GEO) application, was compared statistically. RESULTS Expression of four genes, AGT (angiotensinogen), ENPEP (aminopeptidase A) MME (neprilysin), and PREP (prolyl endopeptidase), was significantly upregulated in CRC specimens. Expression of REN (renin), THOP (thimet oligopeptidase), NLN (neurolysin), PRCP (prolyl carboxypeptidase), ANPEP (aminopeptidase N), and MAS1 (Mas receptor) was downregulated in CRC specimens. CONCLUSIONS Presuming gene expression parallel protein expression, these results suggest that increased production of the angiotensinogen precursor of angiotensin (ANG) peptides, with the reduction of the enzymes that metabolize it to ANG II, can lead to accumulation of angiotensinogen in CRC tissues. Downregulation of THOP, NLN, PRCP, and MAS1 gene expression, whose proteins contribute to the ACE2/ANG 1-7/Mas axis, suggests that reduced activity of this RAS branch could be permissive for oncogenicity. Components of the RAS may be potential therapeutic targets for treatment of CRC.

中文翻译：

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肾素-血管紧张素系统成分和相关蛋白在结直肠癌中的基因表达变化。

材料和方法 使用来自公共功能基因组数据存储库、Gene Expression Omnibus (GEO) 应用程序的芯片阵列获得的正常和癌变组织中这 17 个基因的 RNA 的定量表达进行了统计学比较。结果 CRC 标本中 AGT（血管紧张素原）、ENPEP（氨肽酶 A）、MME（脑啡肽酶）和 PREP（脯氨酰内肽酶）四种基因的表达显着上调。REN（肾素）、THOP（硫柳肽寡肽酶）、NLN（溶神经素）、PRCP（脯氨酰羧肽酶）、ANPEP（氨肽酶 N）和 MAS1（Mas 受体）的表达在 CRC 标本中下调。结论 假设基因表达平行蛋白表达，这些结果表明血管紧张素 (ANG) 肽的血管紧张素原前体的产生增加，随着将其代谢为 ANG II 的酶的减少，可导致 CRC 组织中血管紧张素原的积累。THOP、NLN、PRCP 和 MAS1 基因表达的下调，其蛋白质有助于 ACE2/ANG 1-7/Mas 轴，表明该 RAS 分支的活性降低可能允许致癌性。RAS 的成分可能是治疗 CRC 的潜在治疗靶点。