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Digital camera analysis of dichlorvos by phloroglucinol and quantitate with standard colour chart in environmental water matrices – an approach
Indian Journal of Chemistry, Section A ( IF 0.412 ) Pub Date : 2021-07-30
Kamesh Viswanathan Baskaran

Dichlorvos (DDVP) analysis using visible spectrophotometer, gas chromatography-mass spectrometry and liquid chromatography mass spectrometry has been studied extensively. The major drawback of all the method is requirement of standardisation, expensive and maintenance nature of instrument involvement. Since, many governments has banned the use of DDVP, standardisation is quite challenging with single point standard method. In this study, using digital camera based colour analysis for DDVP is carried out to develop one-time multistandard colour chart. The analysis is carried out by alkaline degradation of DDVP to dichloroacetylaldehyde to react with phloroglucinol and producing distinct colour gradients from pale purple to dark brown. The colour chart is able to quantitate the limit of detection and limit of quantification of 6 μg/mL and 20 μg/mL, respectively. The study is further experimented with real-time ground water with non-spiked and spiked DDVP samples for pre-concentration effect. The 1:1 linear relation is found with absorbance based spectrophotometer detection and digital camera image analysis to quantify around ppb level. This shows the colour analysis by human naked eye judgement is possible at certain level as semi-quantitate by strategical approach. In this paper, we have used the colour chart based digital camera detection of DDVP, which will be a viable alternate approach for analysing the environment samples.

中文翻译:

用间苯三酚对敌敌畏进行数码相机分析,并使用环境水基质中的标准比色图进行定量——一种方法

使用可见光分光光度计、气相色谱-质谱法和液相色谱质谱法进行敌敌畏 (DDVP) 分析已得到广泛研究。所有方法的主要缺点是需要标准化、昂贵和仪器涉及的维护性质。由于许多政府已禁止使用 DDVP,因此单点标准方法的标准化非常具有挑战性。在这项研究中,使用基于数码相机的 DDVP 颜色分析进行了开发一次性多标准颜色图表。该分析是通过 DDVP 碱性降解成二氯乙酰醛与间苯三酚反应并产生从浅紫色到深棕色的不同颜色梯度来进行的。比色图能够定量检测限和定量限为 6 μg/mL 和 20 μg/mL,分别。该研究进一步对实时地下水进行了实验,其中包含未加标和加标的 DDVP 样品,以实现预浓缩效果。通过基于吸光度的分光光度计检测和数码相机图像分析发现 1:1 线性关系,以量化 ppb 级左右。这说明通过人的肉眼判断颜色分析在一定程度上是可以通过策略方法进行半定量的。在本文中,我们使用了基于彩色图表的 DDVP 数码相机检测,这将是分析环境样本的一种可行的替代方法。通过基于吸光度的分光光度计检测和数码相机图像分析发现了 1 线性关系,以量化 ppb 级左右。这说明通过人的肉眼判断颜色分析在一定程度上是可以通过策略方法进行半定量的。在本文中,我们使用了基于彩色图表的 DDVP 数码相机检测,这将是分析环境样本的一种可行的替代方法。通过基于吸光度的分光光度计检测和数码相机图像分析发现了 1 线性关系,以量化 ppb 级左右。这说明通过人的肉眼判断颜色分析在一定程度上是可以通过策略方法进行半定量的。在本文中,我们使用了基于彩色图表的 DDVP 数码相机检测,这将是分析环境样本的一种可行的替代方法。
更新日期:2021-07-30
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