The sensitivity of pigs to deoxynivalenol (DON) might be increased by systemic inflammation (SI), which also has consequences for hepatic integrity. Liver lesions and a dys-regulated gene network might hamper hepatic handling and elimination of DON whereby the way of initiation of hepatic inflammation might play an additional role. First and second-pass exposure of the liver with LPS for triggering a SI was achieved by LPS infusion via pre- or post-hepatic venous route, respectively. Each infusion group was pre-conditioned either with a control diet (0.12 mg DON/kg diet) or with a DON-contaminated diet (4.59 mg DON/kg diet) for 4 wk. Liver transcriptome was evaluated at 195 min after starting infusions. DON exposure alone failed to modulate the mRNA expression significantly. However, pre- and post-hepatic LPS challenges prompted transcriptional responses in immune and metabolic levels. The mRNAs for B-cell lymphoma 2-like protein 11 as a key factor in apoptosis and IFN-γ released by T cells were clearly up-regulated in DON-fed group infused with LPS post-hepatically. On the other hand, mRNAs for nucleotide binding oligomerization domain containing 2, IFN-α and eukaryotic translation initiation factor 2α kinase 3 as ribosomal stress sensors were exclusively up-regulated in control pigs with pre-hepatic LPS infusion. These diverse effects were traced back to differences in TLR4 signalling.

全身炎症 (SI) 可能会增加猪对脱氧雪腐镰刀菌烯醇 (DON) 的敏感性，这也会对肝脏完整性产生影响。肝脏病变和失调的基因网络可能会阻碍肝脏处理和消除 DON，从而引发肝脏炎症的方式可能发挥额外的作用。通过 LPS 输注通过LPS 对肝脏进行第一次和第二次暴露以触发 SI肝前或肝后静脉途径，分别。每个输注组用对照饮食（0.12 mg DON/kg 饮食）或用 DON 污染饮食（4.59 mg DON/kg 饮食）预处理 4 周。在开始输注后 195 分钟评估肝转录组。单独的 DON 暴露未能显着调节 mRNA 表达。然而，肝前和肝后 LPS 挑战促使免疫和代谢水平的转录反应。B 细胞淋巴瘤 2 样蛋白 11 的 mRNA 作为细胞凋亡的关键因素和 T 细胞释放的 IFN-γ 在肝后输注 LPS 的 DON 喂养组中明显上调。另一方面，核苷酸结合寡聚结构域的 mRNAs 含有 2，作为核糖体应激传感器的 IFN-α 和真核翻译起始因子 2α 激酶 3 在肝前 LPS 输注的对照猪中完全上调。这些不同的影响可以追溯到 TLR4 信号传导的差异。