Advances in neuroscience have relied on the development of techniques that examine neuronal cell activities. One major challenge involves the limitations in labeling and controlling neuronal activities relating to the cell's activation state. In this study, the modified human codon-optimized channelrhodopsin-2 photoreceptor hChR2(C128S) was integrated into function with inducible gene expression methods and materials: the Tet system and the highly efficient minimum promoter of Arc/Arg3.1. The system successfully expressed the target fusion gene exclusively in activated SH-SY5Y human neuroblastoma cells while maintaining the essential characteristics of ChR2. The expression of the channelrhodopsin construct was observed, while the expression duration was refined by treatment with doxycycline. The optogenetic construct here tested the application of the minimum Arc/Arg3.1 promoter, an advanced immediate-early gene promoter, for the expression of the channelrhodopsin gene. Along with its noninvasive nature, this expression system promises to serve dual functions as a cell activity indicator and cell actuator, creating the possibility for researchers to precisely label cells according to their activation state and control the activities of specific neuronal cell populations.

神经科学的进步依赖于检查神经元细胞活动的技术的发展。一个主要挑战涉及标记和控制与细胞激活状态相关的神经元活动的限制。在这项研究中，将经过修饰的人密码子优化通道视紫红质-2 光感受器hChR2(C128S) 与可诱导基因表达方法和材料整合到功能中：Tet 系统和Arc/Arg3.1 的高效最小启动子。该系统成功地在激活的 SH-SY5Y 人神经母细胞瘤细胞中表达了靶融合基因，同时保持了 ChR2 的基本特征。观察到通道视紫红质构建体的表达，而通过用强力霉素处理来细化表达持续时间。此处的光遗传学构建体测试了最小 Arc/Arg3.1 启动子（一种先进的即早基因启动子）在通道视紫红质基因表达中的应用。除了其无创性外，该表达系统还有望作为细胞活动指标和细胞执行器发挥双重功能，为研究人员根据其激活状态精确标记细胞并控制特定神经元细胞群的活动创造了可能性。