Background

Data regarding yeast microbiota in goat milk is scarce.

Aims

To isolate and identify species of the genus Candida in milk samples from clinically healthy goats, and evaluate their enzymatic activity and biofilm formation.

Methods

1092 milk samples from clinically healthy goats were collected and processed. The yeast isolates were identified by phenotypic, methods and their enzymatic activity (phospholipase, hemolysin and protease) and biofilm formation evaluated.

Results

We obtained 221 Candida isolates belonging to six species: Candida kefyr (35.7%), Candida guilliermondii (33%), Candida famata (23.5%), Candida glabrata (5.9%), Candida albicans (1.35%) and Candida parapsilosis sensu lato (0.45%). Protease activity was detected in all Candida species while hemolysin activity was only present in C. kefyr, C. guilliermondii, C. famata and C. albicans. Only C. albicans showed phospholipase activity. With the exception of C. parapsilosis sensu lato, all Candida species formed biofilm, with 60.19% of the isolates being poor producers, 9.93% moderate producers, and 1.35% strong producers.

Conclusions

The milk of clinically healthy goats contains several species of the genus Candida that could play a role as opportunistic pathogens in mastitis.

中文翻译：

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从山羊奶中分离的念珠菌属的表征、酶活性和生物膜形成

背景

关于山羊奶中酵母菌群的数据很少。

宗旨

从临床健康山羊的牛奶样品中分离和鉴定念珠菌属的物种，并评估它们的酶活性和生物膜形成。

方法

收集和处理了 1092 份来自临床健康山羊的牛奶样品。通过表型、方法和它们的酶活性（磷脂酶、溶血素和蛋白酶）和生物膜形成来鉴定酵母分离物。

结果

我们获得了221念珠菌分离株属于六种：念珠菌乳酒（35.7％），念珠菌季也蒙（33％），念珠菌famata（23.5％），光滑念珠菌（5.9％），白色念珠菌（1.35％）和近平滑念珠菌 意义上拉托（ 0.45%)。在所有念珠菌物种中都检测到蛋白酶活性，而溶血素活性仅存在于C. kefyr、C. guilliermondii、C. famata和C. albicans 中。只有白色念珠菌表现出磷脂酶活性。除了C. parapsilosis sensu lato 之外，所有念珠菌属形成生物膜，60.19% 的分离株为不良生产者，9.93% 为中等生产者，1.35% 为强生产者。

结论

临床健康山羊的奶中含有几种念珠菌属，它们可能在乳腺炎中起到机会性病原体的作用。