In this study, we have developed a novel biomimetic electrochemical sensor sensitized with functionalized carbon nanotubes using a molecularly imprinted film as a recognition element for the rapid detection of uric acid. Using K₃[Fe(CN)₆] as a probe, uric acid imprinted films on electrodes are characterized by voltammetry measurements and electrochemical impedance spectroscopy. The optimizations of experimental steps are conducted by cyclic voltammetry and differential pulse voltammetry. When the imprinted sensor is immersed in the solution containing a certain concentration of uric acid and incubated for a period of time, the oxidation peak current of K₃[Fe(CN)₆] decreases with the increase of uric acid concentration. Under optimal conditions, the peak current of K₃[Fe(CN)₆] has a good linear relationship with uric acid concentration at range from 0.1 μM to 3.3 μM with the detection limit of 0.03 μM. The proposed sensor shows high selectivity for rapid detection of uric acid in human serum samples.

中文翻译：

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基于功能化碳纳米管修饰电极的分子印迹电化学传感器选择性检测尿酸

在这项研究中，我们开发了一种新型仿生电化学传感器，该传感器使用功能化碳纳米管敏化，使用分子印迹膜作为识别元件，用于快速检测尿酸。使用 K ₃ [Fe(CN) ₆ ] 作为探针，通过伏安法测量和电化学阻抗谱表征电极上的尿酸印迹膜。实验步骤的优化通过循环伏安法和微分脉冲伏安法进行。当印迹传感器浸入含有一定浓度尿酸的溶液中并保温一段时间后，K ₃ [Fe(CN) ₆]随着尿酸浓度的增加而降低。在最佳条件下，K ₃ [Fe(CN) ₆ ]的峰值电流与尿酸浓度在0.1 μM至3.3 μM范围内具有良好的线性关系，检测限为0.03 μM。所提出的传感器显示出快速检测人血清样品中尿酸的高选择性。