Reactive oxygen species (ROS) modulator 1 (ROMO1) is a mitochondrial membrane protein that is essential for the regulation of mitochondrial ROS production and redox sensing. ROMO1 regulates ROS generation within cells and is involved in cellular processes, such as cell proliferation, senescence, and death. Our purpose is to investigates the impact of ROMO1 on the mitochondria during porcine embryogenesis. We found that high expression of ROMO1 was associated with porcine preimplantation embryo development, indicating that ROMO1 may contribute to the progression of embryogenesis. Knockdown of ROMO1 disrupted porcine embryo development and blastocyst quality, thereby inducing ROS production and decreasing mitochondrial membrane potential. Knockdown of ROMO1 induced mitochondrial dysfunction by disrupting the balance of OPA1 isoforms to release cytochrome c, reduce ATP, and induce apoptosis. Meanwhile, ROMO1 overexpression showed similar effects as ROMO1 KD on the embryos. Overexpression of ROMO1 rescued the ROMO1 KD-induced defects in embryo development, mitochondrial fragmentation, and apoptosis. ROMO1 plays a critical role in embryo development by regulating mitochondrial morphology, function, and apoptosis in pigs.

中文翻译：

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ROMO1是猪植入前胚胎发育过程中线粒体代谢所必需的

活性氧 (ROS) 调节剂 1 (ROMO1) 是一种线粒体膜蛋白，对调节线粒体 ROS 产生和氧化还原传感至关重要。ROMO1 调节细胞内 ROS 的生成，并参与细胞过程，如细胞增殖、衰老和死亡。我们的目的是研究 ROMO1 对猪胚胎发生过程中线粒体的影响。我们发现 ROMO1 的高表达与猪植入前胚胎发育有关，表明 ROMO1 可能有助于胚胎发生的进程。敲除 ROMO1 会破坏猪胚胎发育和囊胚质量，从而诱导 ROS 产生并降低线粒体膜电位。敲低 ROMO1 通过破坏 OPA1 异构体的平衡以释放细胞色素 c、减少 ATP 并诱导细胞凋亡，从而诱导线粒体功能障碍。同时，ROMO1 过表达对胚胎显示出与 ROMO1 KD 相似的影响。ROMO1 的过表达挽救了 ROMO1 KD 诱导的胚胎发育、线粒体断裂和细胞凋亡缺陷。ROMO1 通过调节猪的线粒体形态、功能和细胞凋亡在胚胎发育中发挥关键作用。