In this study, a Mn(II)-based coordination polymer with the chemical composition of {[Mn(L)(TBTA)(H2O)2]ꞏH2O}n (1) has been prepared by reacting Mn(OAc)2ꞏ2H2O with the flexible ligand 1,4-bis(5,6-dimethylbenzimidazol-1-yl)-2-butylene in the aid of the carboxylic acid ligand tetrabromoterephthalic acid (H2TBTA) through the mixed-ligand method. Its application value on osteosarcoma has been measured, and the internal principle is discussed simultaneously. The inhibitory activity of the new compound on the multiplication of osteosarcoma cells is evaluated using the Cell Counting Kit-8 detection kit. The facilitation of the LKB1-mTORC1 signalling pathway is estimated via real-time RT-PCR.

中文翻译：

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基于柔性苯并咪唑配体的新型 Mn(II) 配位聚合物：晶体

在本研究中，通过将 Mn(OAc)2ꞏ2H2O 与化学成分 {[Mn(L)(TBTA)(H2O)2]ꞏH2O}n (1)柔性配体1,4-双(5,6-二甲基苯并咪唑-1-基)-2-丁烯在羧酸配体四溴对苯二甲酸(H2TBTA)的帮助下通过混合配体法。测量了其在骨肉瘤中的应用价值，同时探讨了其内在原理。使用 Cell Counting Kit-8 检测试剂盒评估新化合物对骨肉瘤细胞增殖的抑制活性。LKB1-mTORC1 信号通路的促进作用是通过实时 RT-PCR 估计的。