In our study, FISH mapping using 18S-5.8S-25S rDNA and 5S rDNA sequences was performed for the first time on Ophrys tenthredinifera Willdenow, 1805, Serapias vomeracea (Burman f., 1770) Briquet, 1910 and Himantoglossum hircinum (Linnaeus, 1753) Sprengel, 1826. A detailed study was also performed on O. tenthredinifera using Giemsa-staining, silver-staining, CMA fluorescence banding and fluorescence in situ hybridisation (FISH) with rDNA probes. We analysed two subspecies, i.e. O. tenthredinifera subsp. neglecta (Parlatore, 1860) E.G. Camus, 1908 and O. tenthredinifera subsp. grandiflora (Tenore, 1819) Kreutz, 2004 by the traditional Feulgen method and constructed the karyotype. The cytotaxonomic implications for both taxa are also discussed. In Himantoglossum hircinum, FISH and silver staining highlighted differences in the number of two rDNA families (35S and 5S) with respect to Barlia robertiana (Loiseleur-Deslongchamps, 1807) Greuter, 1967. In addition, fluorescence in situ hybridisation was also applied to diploid (2n = 2x = 36) and triploid (2n = 3x = 54) Anacamptis morio (Linnaeus, 1753) R.M. Bateman, Pridgeon et M.W. Chase, 1997. As far as we are aware, this is the first case of autotriploidy observed in A. morio.

中文翻译：

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兰科亚族（兰科）物种的比较染色体研究

 在我们的研究中，首次使用 18S-5.8S-25S rDNA 和 5S rDNA 序列对 Ophrys tenthredinifera Willdenow, 1805, Serapias vomeracea (Burman f., 1770) Briquet, 1910 和 Himantoglossum hircinum (Linnaeus, 1753) 进行 FISH 作图) Sprengel，1826 年。还使用 Giemsa 染色、银染色、CMA 荧光条带和荧光原位杂交 (FISH) 与 rDNA 探针对 O. tenthredinifera 进行了详细研究。我们分析了两个亚种，即 O. tenthredinifera subsp。ignorea (Parlatore, 1860) EG Camus, 1908 和 O. tenthredinifera subsp. grandiflora (Tenore, 1819) Kreutz, 2004 通过传统的 Feulgen 方法构建了核型。还讨论了这两种分类群的细胞分类学意义。在 Himantoglossum hircinum 中，