The bioinformatic analysis that we made of 492 Acinetobacter baumannii plasmid sequences identified 418 genes encoding Replication Initiator (Rep) proteins that fell into at least fourteen groups according to the protein domains that they contained. The most abundant group of Rep proteins contained a Rep_3 superfamily domain, followed by Rep proteins containing Replicase/PriCT_1 superfamily domains, and then by Reps possessing only an HTH_MerR-SF superfamily domain. The remaining eleven groups contain only a few members. To evaluate the diversity of these Rep proteins, we classify them using the current scheme of GR homology groups, which contains 34 groups. However, we needed to create 22 additional GR homology groups to capture all the Rep protein diversity of the plasmid collection. Finally, our bioinformatic analysis suggests that a large fraction of the plasmids seem to have a restricted host range limited to Acinetobacter species, except for those belonging to GR38 that have a very wide host range. To facilitate the future analysis of the Rep proteins, we included a list of the DNA and protein sequences, in fasta format, of the representatives of each one of the GR homology groups.

我们对 492株鲍曼不动杆菌进行的生物信息学分析质粒序列鉴定了 418 个编码复制启动子 (Rep) 蛋白的基因，根据它们所包含的蛋白质结构域，这些基因至少分为 14 组。最丰富的 Rep 蛋白组包含 Rep_3 超家族结构域，其次是包含 Replicase/PriCT_1 超家族结构域的 Rep 蛋白，然后是仅具有 HTH_MerR-SF 超家族结构域的 Rep 蛋白。剩下的 11 个小组只包含几个成员。为了评估这些 Rep 蛋白的多样性，我们使用当前的 GR 同源组方案对它们进行分类，其中包含 34 个组。然而，我们需要创建 22 个额外的 GR 同源组来捕获质粒集合的所有 Rep 蛋白多样性。最后，我们的生物信息学分析表明，大部分质粒似乎具有受限的宿主范围，仅限于不动杆菌属物种，但属于 GR38 的那些具有非常广泛的宿主范围。为了便于未来对 Rep 蛋白的分析，我们以 fasta 格式列出了每个 GR 同源组的代表的 DNA 和蛋白质序列列表。