We used the chloroplast markers trnL-F and rbcL to construct a DNA barcode sequence library of Adiantum (Pteridaceae) from Puerto Rico. By conducting BLAST searches, the trnL-F marker was able to successfully determine specimens of six out of 10 species for which data were generated, and failed for four. For the nine species for which rbcL sequences were obtained, the BLAST search method was successful for three, partially successful for three, and a failure for three. Phylogenetic analyses of both markers were able to resolve and distinguish all sampled taxa, with the exception that sequences of A. latifolium and A. ×variopinnatum were recovered as an unresolved polytomy with A. petiolatum nested within it. The molecular data indicate that what has previously been determined as A. pyramidale on Jamaica, Cuba, Hispaniola, and Puerto Rico likely represents at least two distinct species, based on data from Cuba and Puerto Rico. We also report the presence of A. ×variopinnatum on Puerto Rico for the first time.

中文翻译：

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波多黎各铁线蕨（Pteridaceae：Vittarioideae）的 DNA 条形码

我们使用叶绿体标记 trnL-F 和 rbcL 构建了来自波多黎各的铁线蕨 (Pteridaceae) 的 DNA 条形码序列库。通过进行 BLAST 搜索，trnL-F 标记能够成功地确定 10 个物种中的 6 个已生成数据的样本，但失败了 4 个。对于获得 rbcL 序列的 9 个物种，BLAST 搜索方法成功了三个，部分成功了三个，失败了三个。两种标记的系统发育分析都能够解析和区分所有采样的分类群，除了 A. latifolium 和 A. ×variopinnatum 的序列被恢复为未解析的多聚体，其中 A. petiolatum 嵌套在其中。分子数据表明，以前在牙买加、古巴、伊斯帕尼奥拉岛、根据古巴和波多黎各的数据，波多黎各可能代表至少两个不同的物种。我们还首次报告了波多黎各存在 A. ×variopinnatum。