Abstract

In some cases standard chemotherapy of acute lymphocytic leukemia (ALL) leads to neurotoxicity; its mechanisms, methods of prognosis, and prevention are being actively studied. The aim of this study was to assess the cerebrospinal fluid (CSF) cytokine profile in children with ALL and neurotoxic side effects of chemotherapy. This prospective study included 24 children with ALL aged from 3 to 17 years. Patients were further subdivided into ALL patients with (the main group) and without neurological complications (the comparison group). The level of CSF cytokines was measured by the Xmap technology (Luminex) using Invitrogen test systems (eBioscience) and the Luminex 200 system. The comparative analysis of the cytokine profile in the group of children with chemotherapy-induced neurotoxic complications revealed elevated levels of chemokine CXCL12 (SDF-1α) and stem cell factor (SCF). The increased level of these cytokines in CSF suggested a relatively risk for development of the toxic peripheral neuropathy.

中文翻译：

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急性淋巴细胞白血病患儿脑脊液细胞因子谱及化疗神经毒副作用的研究

摘要

在某些情况下，急性淋巴细胞白血病 (ALL) 的标准化疗会导致神经毒性；目前正在积极研究其发病机制、预后方法和预防方法。本研究的目的是评估患有 ALL 和化疗的神经毒性副作用的儿童的脑脊液 (CSF) 细胞因子谱。这项前瞻性研究包括 24 名 3 至 17 岁的 ALL 儿童。患者进一步细分为有（主要组）和无神经系统并发症（对照组）的ALL患者。使用 Invitrogen 测试系统 (eBioscience) 和 Luminex 200 系统通过 Xmap 技术 (Luminex) 测量 CSF 细胞因子的水平。对化疗引起的神经毒性并发症儿童组细胞因子谱的比较分析显示，趋化因子 CXCL12 (SDF-1α) 和干细胞因子 (SCF) 水平升高。脑脊液中这些细胞因子水平的升高表明发生中毒性周围神经病变的风险相对较高。