Abstract

The post-genomic era is characterized by a range of high throughput profiling methods capable of broadly characterizing gene expression levels, protein, and metabolite abundances. Application of these methods, enzyme profiling, and more recently, protein-metabolite interactions and flux analysis have alongside modeling approaches allowed us to refine our understanding of the regulation of metabolism even in the case of the canonical pathways of primary plant metabolism. Here we review recent insights obtained by using such methods in the context of our previous knowledge. In doing so, we hope to highlight the effectiveness of these methods and postulate that their application to less well-studied metabolic pathways will likely allow the elucidation of the hitherto unknown mechanism of metabolic regulation.

摘要

后基因组时代的特点是一系列高通量分析方法能够广泛表征基因表达水平、蛋白质和代谢物丰度。这些方法的应用、酶谱分析以及最近的蛋白质-代谢物相互作用和通量分析与建模方法一起使我们能够改进对代谢调节的理解，即使在植物初级代谢的典型途径的情况下也是如此。在这里，我们回顾了在我们先前知识的背景下使用这些方法获得的最新见解。在此过程中，我们希望强调这些方法的有效性，并假设将它们应用于研究较少的代谢途径可能有助于阐明迄今为止未知的代谢调节机制。