Abstract

S-Equol is an intestinal bacterial metabolite of the isoflavone daidzein. Racemic (±)-equol is used as a dietary estrogen supplement in menopausal women and is suggested to affect the female reproductive function and development, primarily controlled by the hypothalamic-pituitary-gonadal axis and gonadotropin-releasing hormone release. In this study, murine neurons were utilized to evaluate the effect and mechanism of (±)-equol on the secretion of gonadotropin-releasing hormone. The cells were treated with up to 20 µmol/L of (±)-equol to determine the influence on cell viability. Enzyme-linked immunosorbent assay, real-time quantitative polymerase chain reaction, and Western-blotting analysis were used to investigate the impacts of (±)-equol on the secretion and transcription of gonadotropin-releasing hormone, and the transcription and translation of key neuromodulators. This study showed that (±)-equol could decrease cell viability, increase the ability to secrete gonadotropin-releasing hormone, and interfere with the expression of neurokinin B receptor, makorin ring finger protein 3, and silent information regulator 1.

摘要

S-Equol 是异黄酮黄豆苷元的肠道细菌代谢产物。外消旋（±）-牛尿酚用作绝经期妇女的膳食雌激素补充剂，建议影响女性生殖功能和发育，主要受下丘脑-垂体-性腺轴和促性腺激素释放激素释放的控制。本研究利用小鼠神经元评价( ± )-牛尿酚对促性腺激素释放激素分泌的影响及机制。用高达 20 µmol/L 的 ( ± )-牛尿酚处理细胞以确定对细胞活力的影响。酶联免疫吸附试验、实时定量聚合酶链反应和蛋白质印迹分析用于研究 ( ±)-牛尿酚对促性腺激素释放激素的分泌和转录，以及关键神经调节剂的转录和翻译。本研究表明，( ± )-牛尿酚可降低细胞活力，增加促性腺激素释放激素的分泌能力，并干扰神经激肽 B 受体、makorin 无名指蛋白 3 和沉默信息调节因子 1 的表达。