Marine seaweeds are known to have a potential role against microbial and pesticidal activities. Ulva lactuca, a green macroalgae extract analysed through gas chromatography mass spectrometry reveals 31 compounds. Resistance of mosquito vectors to synthetic insecticides remains a major problem. Discovering and applying natural agents to act against disease vectors is challenging. The activities of the extract and nano-fabricated green synthesised silver nanoparticles were checked for use against Aedes aegypti and Culex pipiens. The crude extract and synthesised silver nanoparticles exhibited a notable larvicidal effect, and very effective inhibition of pupal and adult emergence. Inhibition of adult emergence of Ae.aegypti was 97.7% and in Cu.pipiens, it was 93.3%. Our genotypic study of Deoxyribonucleic acid from treated larvae utilising random primers MA-09, MA-12 and MA-26 revealed damaged nucleotide sequences when compared with the controls. The antimicrobial activity of both the extract and green synthesised nanomaterials showed prominent activity against pathogenic drug resistant bacteria. Our results contribute to further development of eco-friendly insecticides with lower cost of preparation. This could further contribute to further research helping future generations to be free from these deadly disease-causing vectors and pathogenic microbes.

中文翻译：

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石莼和绿色银纳米粒子对蚊子媒介的毒性及其对登革热媒介埃及伊蚊基因组 DNA 的影响。

众所周知，海洋海藻具有对抗微生物和杀虫活性的潜在作用。石莼是一种绿色大型藻类提取物，通过气相色谱质谱分析显示出 31 种化合物。蚊媒对合成杀虫剂的抗药性仍然是一个主要问题。发现并应用天然药物来对抗疾病媒介具有挑战性。检查提取物和纳米制造的绿色合成银纳米颗粒的活性，以用于对抗埃及伊蚊和淡色库蚊。粗提物和合成的纳米银表现出显着的杀幼虫作用，并且对蛹和成虫的羽化具有非常有效的抑制作用。对埃及伊蚊成虫羽化的抑制率为97.7%，对淡皮伊蚊成虫羽化的抑制率为93.3%。我们利用随机引物 MA-09、MA-12 和 MA-26 对处理过的幼虫的脱氧核糖核酸进行基因型研究，结果显示与对照相比，核苷酸序列受损。提取物和绿色合成纳米材料的抗菌活性对致病性耐药细菌均表现出显着的活性。我们的研究结果有助于进一步开发制备成本更低的环保杀虫剂。这可能进一步有助于进一步的研究，帮助后代摆脱这些致命的致病媒介和病原微生物。