Age-related cataracts (ARC) are the leading cause of visual impairment and blindness, affecting 16 million subjects globally. This work aimed to investigate the correlation of serum homocysteine (Hcy), folate, vitamin B6 (VitB6) and ARC. We prospectively enrolled 60 ARC, and 58 age-matched healthy controls in this study. The serum concentrations of Hcy were determined using a fully automatic biochemical analyzer and folate/VitB6 by enzyme-linked immunosorbent assay (ELISA). The diagnostic performance of serum Hcy, folate and VitB6 for ARC were evaluated by receiver operating characteristics (ROC). The mean serum levels of Hcy, folate and VitB6 from the control group were 9.8 ± 2.1 μmol/L, 17.4 ± 2.3 nmol/L, 42.3 ± 5.7 pmol/L, respectively. In comparison, the mean serum levels of Hcy, folate and VitB6 from the ARC group were 12.2 ± 2.5 μmol/L, 15.3 ± 2.6 nmol/L, 40.3 ± 5.1 pmol/L, respectively. Significant statistical difference (p<0.05) were found between the control and ARC groups. The diagnostic sensitivity, specificity and AUC of serum Hcy as a biomarker for ARC were 53.1%, 76.3% and 0.66 (95% CI:0.61-0.76), respectively, which were superior to that of serum folate and VitB6. Serum Hcy was significantly elevated in ARC patients and correlated with ARC development, thus may be used as a serological marker for ARC diagnosis.

中文翻译：

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血清同型半胱氨酸、叶酸、维生素B6与年龄相关性白内障的相关性

年龄相关性白内障 (ARC) 是导致视力障碍和失明的主要原因，影响全球 1600 万受试者。本工作旨在研究血清同型半胱氨酸 (Hcy)、叶酸、维生素 B6 (VitB6) 和 ARC 的相关性。我们在本研究中前瞻性地招募了 60 名 ARC 和 58 名年龄匹配的健康对照。采用全自动生化分析仪测定血清 Hcy 浓度，采用酶联免疫吸附试验 (ELISA) 测定叶酸/VitB6。通过受试者工作特征（ROC）评估血清 Hcy、叶酸和 VitB6 对 ARC 的诊断性能。对照组的平均血清 Hcy、叶酸和 VitB6 水平分别为 9.8 ± 2.1 μmol/L、17.4 ± 2.3 nmol/L、42.3 ± 5.7 pmol/L。相比之下，ARC组的平均血清Hcy、叶酸和VitB6水平为12.2±2.5 μmol/L，15。分别为 3 ± 2.6 nmol/L、40.3 ± 5.1 pmol/L。在对照组和ARC组之间发现了显着的统计学差异（p<0.05）。血清Hcy作为ARC生物标志物的诊断敏感性、特异性和AUC分别为53.1%、76.3%和0.66（95% CI：0.61-0.76），优于血清叶酸和VitB6。ARC患者血清Hcy显着升高并与ARC发展相关，因此可作为ARC诊断的血清学标志物。