Despite the fact that carbamates and organophosphates cause acute poisoning via different mechanisms and require disparate management, they are indistinguishable by current clinical assays. Herein, direct immersion solid-phase microextraction (DI-SPME) plus thermal desorption-electrospray ionization tandem mass spectrometry (TD-ESI/MS/MS) was developed to discern them. Both pesticides spiked in human serum were extracted by SPME and analyzed by TD-ESI/MS/MS. This is a promising emergency care platform as rapid analyses could be done in tiny sample volumes with satisfactory recovery (89.46%-116.32%), precision (covariance <20%), sensitivity (LOD <0.1 μg/mL), turnaround time (<5 minutes), and linearity (R2 = 0.9827-0.9992) within 0.1-100 μg/mL.

中文翻译：

---

直接浸没固相微萃取结合环境电离串联质谱法快速区分血清中的农药用于紧急诊断。

尽管氨基甲酸酯和有机磷酸酯通过不同的机制引起急性中毒并且需要不同的管理，但目前的临床试验无法区分它们。在此，开发了直接浸没固相微萃取 (DI-SPME) 加热解吸-电喷雾电离串联质谱 (TD-ESI/MS/MS) 来辨别它们。人血清中加标的两种农药均通过 SPME 提取并通过 TD-ESI/MS/MS 进行分析。这是一个很有前途的急救平台，因为可以在微小的样本量中进行快速分析，并具有令人满意的回收率 (89.46%-116.32%)、精确度（协方差 <20%）、灵敏度（LOD <0.1 μg/mL）、周转时间（< 5 分钟），线性度（R2 = 0.9827-0.9992）在 0.1-100 μg/mL 范围内。