Abstract

Purpose: The aim of this study was to assess the antibacterial potential and ex vivo skin permeation kinetics of cefixime from bionanocomposite films. Methods: The films were prepared by solvent casting method by using chitosan and starch. The fabricated films were tested for their antibacterial potential against three bacteria i.e. Escherichia coli, Klebsiella pneumonia, and Acetobacter aceti. In vitro permeation studies of cefixime from the films across rat skin was conducted using Franz diffusion cell. Results: The highest antibacterial effect was exhibited by F5 formulation (non-irradiated film) against Escherichia coli and Klebsiella pneumonia; however, antibacterial activity of the films was significantly (p < 0.05) reduced after their irradiation. F5 formulation showed the highest cumulative amount of permeated drug after 24 h, while F1 (100% chitosan) showed the lowest amount of permeated drug. Non-Fickian diffusion (anomalous) was the main mode of drug release from all films. The cross-linking of films by γ-radiations improved their mechanical properties. The percentage swelling ratio was the highest in non-irradiated films having a polymeric blend (50:50). Water uptake of irradiated films was appreciably reduced as compared to non-irradiated films. Conclusion: The synthesized bionanocomposites are promising therapeutic moieties which not only improve drug permeability across but also ameliorates antibacterial potential of cefixime.

摘要

目的：本研究的目的是评估头孢克肟从生物纳米复合薄膜中的抗菌潜力和离体皮肤渗透动力学。方法：以壳聚糖和淀粉为原料，采用溶剂流延法制备薄膜。测试了制造的薄膜对三种细菌（即大肠杆菌、肺炎克雷伯菌和醋酸醋杆菌）的抗菌潜力。使用 Franz 扩散池对薄膜中的头孢克肟在大鼠皮肤上的体外渗透性研究进行了研究。结果： F5制剂（非辐照薄膜）对大肠杆菌的抗菌效果最高和肺炎克雷伯菌；然而，薄膜的抗菌活性在照射后显着降低（p  < 0.05）。F5 制剂在 24 小时后显示出最高的累积渗透药物量，而 F1（100% 壳聚糖）显示出最低的渗透药物量。非 Fickian 扩散（异常）是所有薄膜药物释放的主要模式。γ-辐射对薄膜的交联改善了它们的机械性能。在具有聚合物共混物 (50:50) 的未辐照薄膜中溶胀百分比最高。与未辐照薄膜相比，辐照薄膜的吸水量明显减少。结论：合成的生物纳米复合材料是有前途的治疗部分，不仅可以提高药物的渗透性，还可以改善头孢克肟的抗菌潜力。