Abstract The aim of this study was to evaluate the potential inhibitory effect of montmorillonite (MMT)-reinforced, glutaraldehyde-crosslinked PVA (polyvinyl alcohol) nanofibers loaded with root extract of Berberis lycium on monoamine oxidase A and B (MAO A and B) and butyrylcholinesterase (BChE) by using slightly modified Ellman’s test and Amplex Red monoamine oxidase assay, respectively. Enzyme inhibition studies of extract-loaded nanofibers showed significant inhibitory potential against MAO A, B, and BChE. There was an increase in enzyme inhibition with an increased extract concentration loaded to nanofibers. The fibers were characterized by TGA (thermal gravimetric analysis), SEM (scanning electron microscopy), XRD (X-ray diffractometry), and FTIR (Fourier-transform infra-red) spectroscopy to investigate thermal stability, morphology, structural changes, and functional groups in the nanofibers, respectively. SEM results of fabricated nanofibers reflected the beadless and smooth morphology of nanofibers with the porous structure. The contact angle measurements of fabricated nanofibers showed suitable hydrophilicity of nanofibers. The nanofibers loaded with the root extract of Berberis lycium have been found to be potent inhibitors of MAO A, B, and the BChE enzyme.

中文翻译：

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PVA-小檗枸杞纳米纤维的开发及丁酰胆碱酯酶/单胺氧化酶抑制潜力

摘要 本研究的目的是评估负载小檗根提取物的蒙脱石 (MMT) 增强的戊二醛交联 PVA（聚乙烯醇）纳米纤维对单胺氧化酶 A 和 B（MAO A 和 B）和丁酰胆碱酯酶 (BChE) 分别使用略微修改的 Ellman 试验和 Amplex Red 单胺氧化酶测定法。负载提取物的纳米纤维的酶抑制研究显示出对 MAO A、B 和 BChE 的显着抑制潜力。随着加载到纳米纤维上的提取物浓度的增加，酶抑制也增加了。通过 TGA（热重分析）、SEM（扫描电子显微镜）、XRD（X 射线衍射）和 FTIR（傅里叶变换红外）光谱对纤维进行表征，以研究热稳定性，纳米纤维的形态、结构变化和官能团。制备的纳米纤维的SEM结果反映了具有多孔结构的纳米纤维的无珠和光滑形态。制备的纳米纤维的接触角测量显示了纳米纤维合适的亲水性。载有小檗根提取物的纳米纤维已被发现是 MAO A、B 和 BChE 酶的有效抑制剂。