Abstract

BACKGROUND:

Blueberry production has generated great commercial expectations, therefore for its agricultural expansion it is necessary to overcome the challenges at the time of mass propagation.

OBJECTIVE:

Evaluate the effect of a set of factors influencing micropropagation, as well as the influence of substrates on the ex vitro morpho-physiological performance of blueberry seedlings.

METHODS:

A set of protocols were developed to optimize all stages of micropropagation (aseptic establishment, multiplication, rooting, and acclimatization) of blueberries.

RESULTS:

Explants immersed in 1.5% NaClO for 8 min and then in 0.1% HgCl₂ for 2 min achieved 100% sterility and a viability rate of 86.67% for ‘Biloxi’ and 93.33% for ‘Bluecrop’. At the multiplication stage, the maximum number of shoots of ‘Biloxi’ (3.53) and ‘Bluecrop’ (2.27) were obtained on the medium supplemented with 0.2 and 10 mg L^–1 silver nanoparticles (AgNPs), respectively. The percentage of in vitro rooting was significantly improved on media containing activated charcoal, with levels between 80% and 100% . In the acclimatization phase, plants grown in a substrate composed of peat and cocomix® (2:1 ratio) showed greater uniformity and better morpho-physiological behavior.

CONCLUSIONS:

The present results could be successfully used for large-scale commercial production of blueberries of the varieties ‘Biloxi’ and ‘Bluecrop’.

中文翻译：

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影响'Bluecrop'和'Biloxi'蓝莓微繁的优化因素及其体外驯化过程中形态生理特征的评价

摘要

背景：

蓝莓生产已经产生了巨大的商业期望，因此其农业扩张有必要克服大规模繁殖时的挑战。

客观的：

评估影响微繁殖的一组因素的影响，以及底物对蓝莓幼苗体外形态生理性能的影响。

方法：

开发了一套协议来优化蓝莓微繁殖的所有阶段（无菌建立、繁殖、生根和驯化）。

结果：

外植体在 1.5% NaClO 中浸泡 8 分钟，然后在 0.1% HgCl ₂中浸泡 2 分钟，达到 100% 无菌，'Biloxi' 和 'Bluecrop' 的存活率为 86.67% 和 93.33%。^{在繁殖阶段，'Biloxi' (3.53) 和'Bluecrop' (2.27) 在分别添加了 0.2 和 10 mg L -1}银纳米粒子 (AgNPs)的培养基上获得了最大数量的枝条。在含有活性炭的培养基上，体外生根的百分比显着提高，水平在 80% 和 100% 之间。在驯化阶段，在由泥炭和 cocomix®（2:1 比例）组成的基质中生长的植物表现出更高的均匀性和更好的形态生理行为。

结论：

目前的结果可以成功地用于“Biloxi”和“Bluecrop”品种蓝莓的大规模商业生产。