ABSTRACT

Introduction

We report the results of an international bryological expedition to the Khamar-Daban mountain range in Russian southern Siberia. The novel approach of integrative floristics is introduced here as a method of surveying the flora of a region by using a combination of morphological and molecular evidence to identify and verify unexpected findings. We show that use of this approach can deliver a high level of credibility in cases in which direct molecular comparison is possible or the relevant data can be easily obtained from reliable studies informing recent treatments. Integrative floristics can also be used to identify potential taxonomic problems requiring further study.

Methods

Conventional identification methods based on plant anatomy and morphology were supplemented with molecular barcoding (nuclear ITS, plastid trnL–trnF, rps4 and atpB–rbcL regions) of specimens that either did not fit existing morphological concepts perfectly or for which records were unexpected based on previously established phytogeographical patterns.

Key results and conclusions

In total, we recorded ca 380 species of mosses and ca 150 species of liverworts from five localities on the northern slopes of the Khamar-Daban range. Such floristic richness indicates a hotspot of bryophyte diversity with a remarkably high proportion of suboceanic species, such as Brachydontium trichodes (F.Weber) Milde, Herzogiella striatella (Brid.) Z.Iwats. and Racomitrium macounii subsp. alpinum (E.Lawton) Frisvoll. Nearly 20% of specimens were identified or checked using DNA barcoding. This approach showed the presence of previously unrecorded species in a number of genera, including Didymodon, Neckera and Pseudohygrohypnum.

中文翻译：

---

综合植物区系：分子时代生物多样性调查的现代方法，应用于俄罗斯西伯利亚南部哈马尔-达班山脉的探险

摘要

介绍

我们报告了俄罗斯西伯利亚南部哈马尔-达班山脉的国际苔藓学考察的结果。这里介绍了综合植物区系的新方法，作为一种通过结合形态学和分子学证据来识别和验证意外发现的方法来调查一个地区的植物区系。我们表明，在可以进行直接分子比较的情况下，使用这种方法可以提供高水平的可信度，或者可以从可靠的研究中轻松获得相关数据，从而为最近的治疗提供信息。综合植物区系也可用于识别需要进一步研究的潜在分类问题。

方法

基于植物解剖学和形态学的常规鉴定方法辅以分子条形码（核 ITS、质体trn L - trn F、rps 4 和atp B - rbc L 区域），这些标本要么不完全符合现有的形态学概念，要么记录基于先前建立的植物地理模式是出乎意料的。

主要结果和结论

我们总共记录了来自哈马尔-达班山脉北坡五个地点的约 380 种苔藓和约 150 种地钱。这种植物区系的丰富性表明苔藓植物多样性的热点，其中亚洋物种的比例非常高，例如Brachydontium trichodes (F.Weber) Milde、Herzogiella striatella (Brid.) Z.Iwats。和Racomitrium macouii亚种。alpinum (E.Lawton) 弗里斯沃尔。近 20% 的样本是使用 DNA 条形码识别或检查的。这种方法表明在许多属中存在以前未记录的物种，包括Didymodon、Neckera和Pseudohygrohypnum。