Diabetes is an independent risk factor for stroke and amplifies inflammation. Diabetic stroke is associated with a higher risk of death and worse neural function. The identification of effective anti-inflammatory molecules with translational advantages is particularly important to promote perioperative neurorestorative effects. Applying molecular hydrogen, we measured blood glucose levels before and after middle cerebral artery occlusion (MCAO), 48-h cerebral oedema and infarct volumes, as well as 28-day weight, survival and neurological function. We also measured the levels of TLR4, NF-κB p65, phosphorylated NF-κB p65, catecholamines, acetylcholine and inflammatory factors. All measurements comprehensively showed the positive effect and translational advantage of molecular hydrogen on diabetic stroke. Molecular hydrogen improved the weight, survival and long-term neurological function of rats with diabetic stroke and alleviated changes in blood glucose levels before and after middle cerebral artery occlusion (MCAO), but no difference in circadian rhythm was observed. Molecular hydrogen inhibited the phosphorylation of NF-κB and significantly reduced inflammation. Molecular hydrogen mediates neurorestorative effects after stroke in diabetic rats. The effect is independent of circadian rhythms, indicating translational advantages. The molecular mechanism is related to the TLR4/NF-κB pathway and inflammation.

Graphical abstract

Molecular hydrogen (H₂) affects outcomes of ischemic stroke with diabetes mellitus (DM).

中文翻译：

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氢分子介导糖尿病大鼠中风后的神经恢复作用：TLR4/NF-κB 炎症通路

糖尿病是中风的独立危险因素，并会加剧炎症。糖尿病中风与较高的死亡风险和较差的神经功能有关。鉴定具有转化优势的有效抗炎分子对于促进围手术期神经恢复效果尤为重要。利用分子氢，我们测量了大脑中动脉闭塞（MCAO）前后的血糖水平、48小时脑水肿和梗塞体积，以及28天体重、存活率和神经功能。我们还测量了 TLR4、NF-κB p65、磷酸化 NF-κB p65、儿茶酚胺、乙酰胆碱和炎症因子的水平。所有测量全面显示了氢分子对糖尿病中风的积极作用和转化优势。氢分子改善了糖尿病卒中大鼠的体重、存活率和长期神经功能，并减轻了大脑中动脉闭塞（MCAO）前后血糖水平的变化，但没有观察到昼夜节律的差异。氢分子抑制 NF-κB 的磷酸化并显着减少炎症。氢分子介导糖尿病大鼠中风后的神经恢复作用。该效应与昼夜节律无关，表明转化优势。其分子机制与TLR4/NF-κB通路及炎症有关。

图形概要

氢分子 (H ₂ ) 影响缺血性中风合并糖尿病 (DM) 的结果。