ABSTRACT

We used chlorophyll fluorescence technology and biochemical methods to analyze the effects of wood vinegar (WV) on the photosynthetic mechanism and reactive oxygen species metabolism of tobacco (Nicotiana tabacum L.) leaves infected by Pseudomonas syringae pv. tabaci (Pst). The results showed that Pst infection reduced the chlorophyll content and the activities of PSII and PSI, which inhibited the normal photosynthesis of tobacco leaves. However, pretreatment with WV alleviated the degradation of chlorophyll. Treatment with WV alleviated the downregulation of core gene expression in PSII and PSI and improved the photosynthetic electron transfer in Pst-infected leaves. The levels of expression of PetE, ATPa and ATPc in the Pst-infected leaves were significantly upregulated when pretreated with WV. Pst infection increased the rate of production of superoxide anions and the contents of hydrogen peroxide. WV pretreatment could eliminate the oxidative damage of Pst-infected leaves by enhancing the activities of peroxidase (POD) and glutathione peroxidase (GPx) and upregulating the levels of expression of the POD2 and GPX2 genes. In conclusion, pretreatment with WV can alleviate the photosynthetic inhibition and oxidative damage of tobacco leaves caused by Pst infection.

摘要

我们采用叶绿素荧光技术和生化方法分析了木醋液（WV）对丁香假单胞菌感染烟草（ Nicotiana tabacum L.）叶片光合机制和活性氧代谢的影响。烟粉虱( Pst )。结果表明，Pst感染降低了叶绿素含量，降低了PSII和PSI的活性，从而抑制了烟叶的正常光合作用。然而，用 WV 预处理减轻了叶绿素的降解。用 WV 处理减轻了 PSII 和 PSI 中核心基因表达的下调，并改善了Pst中的光合电子转移-受感染的叶子。用WV预处理后， Pst感染叶片中PetE、ATPa和ATPc的表达水平显着上调。Pst感染增加了超氧阴离子的产生率和过氧化氢的含量。WV预处理可以通过增强过氧化物酶(POD)和谷胱甘肽过氧化物酶(GPx)的活性和上调POD2和GPX2基因的表达水平来消除Pst感染叶片的氧化损伤。综上所述，WV预处理可以减轻Pst对烟叶的光合抑制和氧化损伤。感染。