Patched (Ptch) is a receptor in the hedgehog signaling pathway, essential for animal development. Our previous study showed that ptch1 gene participates in the maintenance of the male germline and spermatogenesis in Cynoglossus semilaevis (csptch1). In this study, we identified a patched1 gene homolog (csptch1 x1). The csptch1 x1 gene is 5761 bp long, with a 4638 bp coding sequence that encodes 1545 amino acids. The Csptch1 x1 protein has 12 transmembrane regions and sterol-sensing domains and is highly homologous to the csptch1 (91 amino acids difference). Expression pattern analysis showed that csptch1 x1 is expressed in eight different tissues of adult tongue sole, and the expression is significantly higher in tissues of female than that in male tissues. The expression pattern in developmental stages was also analyzed. csptch1 x1 could be detected at the 1-cell stage and was highly expressed at the blastocyst, somite, and blastopore closing stages, implying that it participates in cell differentiation. In ovarian development, the expression of csptch1 x1 was initiated at 20 days after hatching (dah) and was significantly high at 35–50 and 95–150 dah. In situ hybridization showed that csptch1 x1 was predominantly expressed in primordial germ cells, oocytes, and follicular cells, but the expression of the gene was lower in the testis. These results suggest that csptch1 x1 may be mainly involved in female differentiation and ovarian development, different from the role of csptch1 in spermatogenesis.

Patched (Ptch) 是刺猬信号通路中的一种受体，对动物发育至关重要。我们之前的研究表明，ptch 1 基因参与了半滑舌鳎( csptch1 ) 雄性生殖系和精子发生的维持。在这项研究中，我们鉴定了一个patched1基因同源物 ( csptch1 x1 )。csptch1 x1基因全长 5761 bp，编码序列为 4638 bp，编码 1545 个氨基酸。Csptch1 x1 蛋白具有 12 个跨膜区和甾醇感应结构域，与 csptch1 高度同源（91 个氨基酸差异）。表达模式分析表明csptch1 x1在成年舌鳎的8个不同组织中均有表达，在雌性组织中的表达明显高于雄性组织。还分析了发育阶段的表达模式。csptch1 x1可在 1 细胞阶段检测到，在胚泡、体节和胚孔闭合阶段高表达，暗示它参与细胞分化。在卵巢发育中，csptch1 x1的表达在孵化后 20 天（dah）开始表达，在 35-50 天和 95-150 天时显着升高。原位杂交表明csptch1 x1主要在原始生殖细胞、卵母细胞和滤泡细胞中表达，但在睾丸中的表达较低。这些结果表明csptch1 x1可能主要参与雌性分化和卵巢发育，不同于csptch1在精子发生中的作用。