ABSTRACT

Adipogenesis involves complex interactions between transcription and metabolic signalling. Exploration of the developmental characteristics of intramuscular adipocyte will provide targets for enhancing beef cattle marbling without increasing obesity. Few reports have compared bovine perirenal and intramuscular adipocyte transcriptomes using the combined analysis of transcriptomes and lipid metabolism to explore differences in adipogenic characteristics. We identified perirenal preadipocytes (PRA) and intramuscular preadipocytes (IMA) in Qinchuan cattle. We found that IMA were highly prolific in the early stages of adipogenesis, while PRA shows a stronger adipogenic ability in the terminal differentiation. Bovine perirenal and intramuscular adipocytes were detected through the combined analysis of the transcriptome and metabolome. More triglyceride was found to be upregulated in perirenal adipocytes; however, more types and amounts of unsaturated fatty acids were detected in intramuscular adipocytes, including eicosapentaenoic acid (20:5 n-3; EPA) and docosahexaenoic acid (22:6 n-3; DHA). Furthermore, differentially expressed genes in perirenal and intramuscular adipocytes were positively correlated with the eicosanoid, phosphatidylcholine (PC), phosphatidyl ethanolamine (PE), and sphingomyelin contents. Associated differential metabolic pathways included the glycerolipid and glycerophospholipid metabolisms. Our research findings provide a basis for the screening of key metabolic pathways or genes and metabolites involved in intramuscular fat production in cattle.

摘要

脂肪生成涉及转录和代谢信号之间的复杂相互作用。探索肌肉内脂肪细胞的发育特征将为增强肉牛大理石花纹而不增加肥胖提供目标。很少有报道使用转录组和脂质代谢的组合分析来比较牛肾周和肌肉内脂肪细胞转录组，以探索脂肪形成特征的差异。我们鉴定了秦川牛的肾周前脂肪细胞 (PRA) 和肌肉内前脂肪细胞 (IMA)。我们发现 IMA 在脂肪形成的早期阶段具有高产性，而 PRA 在终末分化中表现出更强的脂肪形成能力。通过转录组和代谢组的联合分析检测牛肾周和肌内脂肪细胞。发现更多的甘油三酯在肾周脂肪细胞中上调；然而，在肌肉内脂肪细胞中检测到更多类型和数量的不饱和脂肪酸，包括二十碳五烯酸（20:5 n-3；EPA）和二十二碳六烯酸（22:6 n-3；DHA）。此外，肾周和肌肉脂肪细胞中差异表达的基因与类花生酸、磷脂酰胆碱 (PC)、磷脂酰乙醇胺 (PE) 和鞘磷脂含量呈正相关。相关的差异代谢途径包括甘油脂和甘油磷脂代谢。我们的研究结果为筛选与牛肌肉内脂肪生成有关的关键代谢途径或基因和代谢物提供了基础。在肌肉内脂肪细胞中检测到更多类型和数量的不饱和脂肪酸，包括二十碳五烯酸（20:5 n-3；EPA）和二十二碳六烯酸（22:6 n-3；DHA）。此外，肾周和肌肉脂肪细胞中差异表达的基因与类花生酸、磷脂酰胆碱 (PC)、磷脂酰乙醇胺 (PE) 和鞘磷脂含量呈正相关。相关的差异代谢途径包括甘油脂和甘油磷脂代谢。我们的研究结果为筛选与牛肌肉内脂肪生成有关的关键代谢途径或基因和代谢物提供了基础。在肌肉内脂肪细胞中检测到更多类型和数量的不饱和脂肪酸，包括二十碳五烯酸（20:5 n-3；EPA）和二十二碳六烯酸（22:6 n-3；DHA）。此外，肾周和肌肉脂肪细胞中差异表达的基因与类花生酸、磷脂酰胆碱 (PC)、磷脂酰乙醇胺 (PE) 和鞘磷脂含量呈正相关。相关的差异代谢途径包括甘油脂和甘油磷脂代谢。我们的研究结果为筛选与牛肌肉内脂肪生成有关的关键代谢途径或基因和代谢物提供了基础。肾周和肌肉内脂肪细胞中差异表达的基因与类花生酸、磷脂酰胆碱（PC）、磷脂酰乙醇胺（PE）和鞘磷脂含量呈正相关。相关的差异代谢途径包括甘油脂和甘油磷脂代谢。我们的研究结果为筛选与牛肌肉内脂肪生成有关的关键代谢途径或基因和代谢物提供了基础。肾周和肌肉内脂肪细胞中差异表达的基因与类花生酸、磷脂酰胆碱（PC）、磷脂酰乙醇胺（PE）和鞘磷脂含量呈正相关。相关的差异代谢途径包括甘油脂和甘油磷脂代谢。我们的研究结果为筛选与牛肌肉内脂肪生成有关的关键代谢途径或基因和代谢物提供了基础。