This study aimed to clarify the expression changes and clinical significance of regulatory T (Treg) cells and follicular regulatory T (TFR) cell subsets divided by glycoprotein A repetitions predominant protein (GARP) and T cell factor 1(TCF1) in peripheral blood of patients with chronic HBV infection. The peripheral blood of 26 chronic hepatitis B (CHB) patients, 27 inactive HBsAg carriers and 32 healthy controls were collected and GARP + percentages in Treg and TFR cells were analyzed by flow cytometry. In addition, Treg and TFR cell subsets sorted by CD62L and TCF1 were analyzed and compared. Correlation analyses were performed between Treg and TFR cell subpopulations and clinical parameters as well as cytokine concentrations, including IL-21, IL-10 and TGF-β1 in plasma. Circulating Treg and TFR levels were elevated in CHB patients. Moreover, GARP and TCF1 were up-regulated in circulating Treg and TFR cells of CHB patients. TCF1 + CD62L− Treg cells were increased while TCF1−CD62L + Treg cells were decreased in CHB patients. TCF1 + CD62L− and TCF1-CD62L− TFR cells were increased while TCF1 + CD62L + TFR cells were decreased in CHB patients. TCF1 + CD62L− Treg cells were positively correlated with HBV DNA, ALT and plasma IL-10, while TCF1 + CD62L + TFR cells were negatively correlated with HBV DNA, HBeAg, HBsAg, ALT, AST, T-BIL and positively correlated with plasma IL-21. Treg and TFR subsets sorted by TCF1, CD62L and GARP were changed in CHB patients. Changes in Treg and TFR functional subsets are associated with antiviral immunity in CHB patients.

本研究旨在阐明调节性 T (Treg) 细胞和滤泡调节性 T (TFR) 细胞亚群除以糖蛋白 A 重复优势蛋白 (GARP) 和 T 细胞因子 1 (TCF1) 在患者外周血中的表达变化和临床意义伴有慢性乙肝病毒感染。收集26名慢性乙型肝炎（CHB）患者、27名非活动性HBsAg携带者和32名健康对照者的外周血，并通过流式细胞术分析Treg和TFR细胞中的GARP+百分比。此外，对由 CD62L 和 TCF1 分选的 Treg 和 TFR 细胞亚群进行了分析和比较。在 Treg 和 TFR 细胞亚群与临床参数以及细胞因子浓度（包括血浆中的 IL-21、IL-10 和 TGF-β1）之间进行相关分析。CHB 患者的循环 Treg 和 TFR 水平升高。而且，GARP 和 TCF1 在 CHB 患者的循环 Treg 和 TFR 细胞中上调。CHB 患者的 TCF1+CD62L-Treg 细胞增加，而 TCF1-CD62L+Treg 细胞减少。CHB 患者的 TCF1 + CD62L- 和 TCF1-CD62L- TFR 细胞增加，而 TCF1 + CD62L + TFR 细胞减少。TCF1+CD62L-Treg细胞与HBV DNA、ALT、血浆IL-10呈正相关，而TCF1+CD62L+TFR细胞与HBV DNA、HBeAg、HBsAg、ALT、AST、T-BIL呈负相关，与血浆呈正相关IL-21。按 T​​CF1、CD62L 和 GARP 分类的 Treg 和 TFR 亚群在 CHB 患者中发生了变化。Treg 和 TFR 功能亚群的变化与 CHB 患者的抗病毒免疫有关。CHB 患者的 TCF1+CD62L-Treg 细胞增加，而 TCF1-CD62L+Treg 细胞减少。CHB 患者的 TCF1 + CD62L- 和 TCF1-CD62L- TFR 细胞增加，而 TCF1 + CD62L + TFR 细胞减少。TCF1+CD62L-Treg细胞与HBV DNA、ALT、血浆IL-10呈正相关，而TCF1+CD62L+TFR细胞与HBV DNA、HBeAg、HBsAg、ALT、AST、T-BIL呈负相关，与血浆呈正相关IL-21。按 T​​CF1、CD62L 和 GARP 分类的 Treg 和 TFR 亚群在 CHB 患者中发生了变化。Treg 和 TFR 功能亚群的变化与 CHB 患者的抗病毒免疫有关。CHB 患者的 TCF1+CD62L-Treg 细胞增加，而 TCF1-CD62L+Treg 细胞减少。CHB 患者的 TCF1 + CD62L- 和 TCF1-CD62L- TFR 细胞增加，而 TCF1 + CD62L + TFR 细胞减少。TCF1+CD62L-Treg细胞与HBV DNA、ALT、血浆IL-10呈正相关，而TCF1+CD62L+TFR细胞与HBV DNA、HBeAg、HBsAg、ALT、AST、T-BIL呈负相关，与血浆呈正相关IL-21。按 T​​CF1、CD62L 和 GARP 分类的 Treg 和 TFR 亚群在 CHB 患者中发生了变化。Treg 和 TFR 功能亚群的变化与 CHB 患者的抗病毒免疫有关。TCF1+CD62L-Treg细胞与HBV DNA、ALT、血浆IL-10呈正相关，而TCF1+CD62L+TFR细胞与HBV DNA、HBeAg、HBsAg、ALT、AST、T-BIL呈负相关，与血浆呈正相关IL-21。按 T​​CF1、CD62L 和 GARP 分类的 Treg 和 TFR 亚群在 CHB 患者中发生了变化。Treg 和 TFR 功能亚群的变化与 CHB 患者的抗病毒免疫有关。TCF1+CD62L-Treg细胞与HBV DNA、ALT、血浆IL-10呈正相关，而TCF1+CD62L+TFR细胞与HBV DNA、HBeAg、HBsAg、ALT、AST、T-BIL呈负相关，与血浆呈正相关IL-21。按 T​​CF1、CD62L 和 GARP 分类的 Treg 和 TFR 亚群在 CHB 患者中发生了变化。Treg 和 TFR 功能亚群的变化与 CHB 患者的抗病毒免疫有关。