Royal jelly (RJ) is a creamy white-yellow liquid that is secreted by the mandibular and hypopharyngeal glands of bees to nourish the larvae. RJ has gained increasing interest in recent years owing to its antioxidant potential. However, little is known about adequate RJ dosing and its effects on genetic material. Thus, the aim of this study was to evaluate the in vivo effects of RJ on genotoxicity and mutagenicity induced by the alkylating agent methyl methanesulfonate (MMS). In this study, 3-month-old Swiss albino male mice (N = 66) were divided into 11 groups for experimentation. Experiments were performed by administering lyophilized RJ (150 mg/kg, 300 mg/kg, and 1000 mg/kg) or water via gavage as pre- and posttreatment processes with the alkylating agent MMS. After treatment, blood samples were collected from the mice via an incision at the end of the tail to conduct comet assays at times of 24 h and 48 h posttreatment. The mice were then euthanized to remove the bone marrow for a micronucleus test. Overall, regardless of dose, RJ did not exhibit genotoxic, mutagenic activity and the administration of high doses, mainly in the form of posttreatment, presented antigenotoxic and antimutagenic actions. Further, a dose-response correlation was observed in the RJ posttreatment groups. These results demonstrate that RJ administration was effective in reversing the damage caused by the alkylating agent MMS.

蜂王浆（RJ）是一种乳白色的白黄色液体，由蜜蜂的下颌腺和下咽腺分泌，用于滋养幼虫。由于其抗氧化潜力，RJ 近年来受到越来越多的关注。然而，关于适当的 RJ 剂量及其对遗传物质的影响知之甚少。因此，本研究的目的是评估 RJ 对烷化剂甲磺酸甲酯 (MMS) 诱导的遗传毒性和致突变性的体内影响。在这项研究中，将 3 个月大的瑞士白化雄性小鼠 (N = 66) 分成 11 组进行实验。实验通过使用烷化剂 MMS 作为预处理和后处理过程通过强饲法施用冻干 RJ（150 mg/kg、300 mg/kg 和 1000 mg/kg）或水来进行。治疗后，通过尾部末端的切口从小鼠身上采集血样，在处理后 24 小时和 48 小时进行彗星检测。然后将小鼠安乐死以取出骨髓用于微核试验。总的来说，无论剂量如何，RJ 都没有表现出遗传毒性、诱变活性，并且主要以后处理的形式给予高剂量，呈现出抗原毒性和抗诱变作用。此外，在 RJ 治疗后组中观察到剂量反应相关性。这些结果表明 RJ 给药可有效逆转由烷化剂 MMS 造成的损害。诱变活性和高剂量给药（主要以后处理的形式）呈现出抗原毒性和抗诱变作用。此外，在 RJ 治疗后组中观察到剂量反应相关性。这些结果证明 RJ 给药可有效逆转由烷化剂 MMS 造成的损害。诱变活性和高剂量给药（主要以后处理的形式）呈现出抗原毒性和抗诱变作用。此外，在 RJ 治疗后组中观察到剂量反应相关性。这些结果证明 RJ 给药可有效逆转由烷化剂 MMS 造成的损害。