Haloarchaea from high saline habitats are known to contain industrially important value-added bioactive compounds such as bacteriorhodopsin, carotenoids, lipids, and proteins. The diversity of Haloarchaea from geographically less explored saline habitats has received less attention. In this study isolation and characterization of the biotechnological potential of novel haloarchaea strains from solar saltern, a hypersaline environment in Marakanam, Tamilnadu was carried out. The samples were collected during the pre-monsoon (July) and post-monsoon (January) season and ten different haloarchaea strains were isolated. UV-Vis spectroscopy analysis revealed the bacteriorhodopsin production by all the isolated strains. Further experiments were carried out to estimate the yield of bacteriorhodopsin and to assess their photocurrent activity and biosensor applications. The maximum yield of bacteriorhodopsin was 5.6 mg/L of cultured lysate. This study will contribute to a better understanding on effect of elicitors on bacteriorhodopsin producing different haloarchaea strains and growing knowledge on their biotechnological applications.

中文翻译：

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提高泰米尔纳德邦马拉卡南地区新型盐古细菌菌株的细菌视紫红质产量

已知来自高盐生境的卤古菌含有工业上重要的增值生物活性化合物，如细菌视紫红质、类胡萝卜素、脂质和蛋白质。来自地理上探索较少的盐碱地栖息地的盐古菌的多样性受到的关注较少。在这项研究中，在泰米尔纳德邦马拉卡南的高盐环境中，对来自日晒盐场的新型盐古细菌菌株的生物技术潜力进行了分离和表征。在季风前（7 月）和季风后（1 月）季节收集样品，并分离出十种不同的盐古细菌菌株。紫外-可见光谱分析揭示了所有分离菌株产生的细菌视紫红质。进行了进一步的实验以估计细菌视紫红质的产量并评估它们的光电流活性和生物传感器应用。细菌视紫红质的最大产量为 5.6 mg/L 培养的裂解物。本研究将有助于更好地了解诱导子对产生不同嗜盐古细菌菌株的细菌视紫红质的影响，并增进对其生物技术应用的了解。