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Antibacterial activity of the released metabolites of sea anemone Stichodactyla gigantea (Forskal, 1775) from the coast of South Andaman, India
Indian Journal of Geo-Marine Sciences ( IF 0.5 ) Pub Date : 2022-08-22
N S Heera, J K Mishra, S Sneha, M C Shehin Sadaka

Marine sessile organisms produce unique bioactive metabolites, which render a defensive barrier against microbial threats and increase survivability in the middle of predators. The earlier studies focused on isolated metabolites from marine sources, composed to exhibit antibacterial, antiviral, and cytotoxic properties. The present study aims to evaluate the antibacterial property of the anemone-released metabolites. The crude and released mucoid metabolite obtained from the sea anemone Stichodactyla gigantea (Forskal, 1775) assayed against five human pathogens like Bacillus subtilis (MTCC 121), Listeria monocytogenes (MTCC 839), Staphylococcus aureus (MTCC839), Bacillus cereus (MTCC 443), and Salmonella enterica typhimurium (MTCC 1252). The assay exhibited positive activity against two pathogens, viz. B. subtilis (MTCC 121) and L. monocytogenes (MTCC 839). Based on the demonstrated activity, the released metabolites were purified using Open Column chromatography. The fractions collected were subjected to an antibacterial assay, which showed a high inhibition zone of 39 mm and 23 mm in diameter against B. subtilis and L. monocytogenes. Followingly, the characterization of purified fractions through GC-MS analysis confirmed the presence of 22 compounds. This study reveals the potential power of the released mucoid metabolites against antibiotic-resistive pathogens. Further studies are essential to elucidate the role of endosymbiont's contribution to mucoid production and their responsiveness towards tackling stressed conditions

中文翻译:

印度南安达曼海岸海葵 Stichodactyla gigantea (Forskal, 1775) 释放的代谢物的抗菌活性

海洋固着生物产生独特的生物活性代谢物,为抵御微生物威胁提供防御屏障,并提高在捕食者中间的生存能力。早期的研究集中在从海洋来源中分离出来的代谢物上,这些代谢物具有抗菌、抗病毒和细胞毒性的特性。本研究旨在评估海葵释放的代谢物的抗菌特性。从海葵 Stichodactyla gigantea (Forskal, 1775) 获得的粗制和释放的粘液代谢物对五种人类病原体进行了检测,如枯草芽孢杆菌 (MTCC 121)、单核细胞增生李斯特菌 (MTCC 839)、金黄色葡萄球菌 (MTCC839)、蜡状芽孢杆菌 (MTCC 443)和鼠伤寒沙门氏菌 (MTCC 1252)。该测定对两种病原体表现出阳性活性,即。枯草芽孢杆菌 (MTCC 121) 和 L. 单核细胞增多症(MTCC 839)。基于证明的活性,使用开放柱色谱法纯化释放的代谢物。对收集的级分进行抗菌试验,结果显示对枯草芽孢杆菌和单核细胞增生李斯特菌具有 39 毫米和 23 毫米直径的高抑菌圈。随后,通过 GC-MS 分析对纯化级分的表征证实了 22 种化合物的存在。这项研究揭示了释放的粘液代谢物对抗生素耐药病原体的潜在作用。进一步的研究对于阐明内共生菌对粘液产生的作用及其对应对压力条件的反应至关重要 对收集的级分进行抗菌试验,结果显示对枯草芽孢杆菌和单核细胞增生李斯特菌具有 39 毫米和 23 毫米直径的高抑菌圈。随后,通过 GC-MS 分析对纯化级分的表征证实了 22 种化合物的存在。这项研究揭示了释放的粘液代谢物对抗生素耐药病原体的潜在作用。进一步的研究对于阐明内共生菌对粘液产生的作用及其对应对压力条件的反应至关重要 对收集的级分进行抗菌试验,结果显示对枯草芽孢杆菌和单核细胞增生李斯特菌具有 39 毫米和 23 毫米直径的高抑菌圈。随后,通过 GC-MS 分析对纯化级分的表征证实了 22 种化合物的存在。这项研究揭示了释放的粘液代谢物对抗生素耐药病原体的潜在作用。进一步的研究对于阐明内共生菌对粘液产生的作用及其对应对压力条件的反应至关重要 这项研究揭示了释放的粘液代谢物对抗生素耐药病原体的潜在作用。进一步的研究对于阐明内共生菌对粘液产生的作用及其对应对压力条件的反应至关重要 这项研究揭示了释放的粘液代谢物对抗生素耐药病原体的潜在作用。进一步的研究对于阐明内共生菌对粘液产生的作用及其对应对压力条件的反应至关重要
更新日期:2022-08-22
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