Developing biosynthesis of silver nanoparticles (Ag-NPs) using plant extract is an environmentally friendly method to reduce the use of harmful chemical substances. The green synthesis of Ag-NPs by Lawsonia inermis extract and its cellular toxicity and the antimicrobial effect was studied. The physical and chemical properties of synthesised Ag-NPs were investigated using UV-visible spectroscopy, infrared spectroscopy, X-ray diffraction (XRD), scanning, and transmission electron microscopy. The average size of Ag-NPs was 40 nm. The XRD result shows peaks at 2θ = 38.07°, 44.26°, 64.43°, and 77.35° are related to the FCC structure of Ag-NPs. Cytotoxicity of synthesised nanoparticles was evaluated by MTT toxicity test on breast cancer MCF7 cell line. Observations showed that the effect of cytotoxicity of nanoparticles on the studied cell line depended on concentration and time. The obtained IC50 was considered for cells at a dose of 250 μg/ml. Growth and survival rates decreased exponentially with the dose. Antimicrobial properties of Ag-NPs synthesised with extract were investigated against Escherichia coli, Salmonella typhimurium, Bacillus cereus, and Staphylococcus aureus to calculate the minimum inhibitory concentration and the minimum bactericidal concentration of (MBC). The results showed that the synthesised Ag-NPs and the plant extract have antimicrobial properties. The lowest concentration of Ag-NPs that can inhibit the growth of bacterial strains was 25 μg/ml.

中文翻译：

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使用 Lawsonia inermis 生物合成银纳米粒子及其生物医学应用。

使用植物提取物开发银纳米粒子 (Ag-NPs) 的生物合成是一种减少有害化学物质使用的环保方法。研究了Lawsonia inermis提取物绿色合成Ag-NPs及其细胞毒性和抗菌作用。使用紫外-可见光谱、红外光谱、X 射线衍射 (XRD)、扫描和透射电子显微镜研究合成的 Ag-NPs 的物理和化学性质。Ag-NPs 的平均尺寸为 40 nm。XRD 结果显示 2θ = 38.07°、44.26°、64.43° 和 77.35° 处的峰与 Ag-NPs 的 FCC 结构有关。通过对乳腺癌MCF7细胞系的MTT毒性试验评估合成纳米颗粒的细胞毒性。观察表明，纳米粒子的细胞毒性对所研究细胞系的影响取决于浓度和时间。对于 250 μg/ml 剂量的细胞，考虑获得的 IC50。生长和存活率随剂量呈指数下降。用提取物合成的 Ag-NPs 对大肠杆菌、鼠伤寒沙门氏菌、蜡状芽孢杆菌和金黄色葡萄球菌的抗菌特性进行了研究，以计算 (MBC) 的最低抑菌浓度和最低杀菌浓度。结果表明，合成的Ag-NPs和植物提取物具有抗菌性能。可抑制菌株生长的Ag-NPs最低浓度为25 μg/ml。生长和存活率随剂量呈指数下降。用提取物合成的 Ag-NPs 对大肠杆菌、鼠伤寒沙门氏菌、蜡状芽孢杆菌和金黄色葡萄球菌的抗菌特性进行了研究，以计算 (MBC) 的最低抑菌浓度和最低杀菌浓度。结果表明，合成的Ag-NPs和植物提取物具有抗菌性能。可抑制菌株生长的Ag-NPs最低浓度为25 μg/ml。生长和存活率随剂量呈指数下降。用提取物合成的 Ag-NPs 对大肠杆菌、鼠伤寒沙门氏菌、蜡状芽孢杆菌和金黄色葡萄球菌的抗菌特性进行了研究，以计算 (MBC) 的最低抑菌浓度和最低杀菌浓度。结果表明，合成的Ag-NPs和植物提取物具有抗菌性能。可抑制菌株生长的Ag-NPs最低浓度为25 μg/ml。结果表明，合成的Ag-NPs和植物提取物具有抗菌性能。可抑制菌株生长的Ag-NPs最低浓度为25 μg/ml。结果表明，合成的Ag-NPs和植物提取物具有抗菌性能。可抑制菌株生长的Ag-NPs最低浓度为25 μg/ml。